单细胞测序技术发展及其在作物研究中的应用

Progress of Single Cell Sequencing Technology and Its Application in Crops Research

作物同一组织中的细胞往往被认为是具有相同状态的功能单位,传统的检测手段分析的是细胞群体的总体平均反应。而单细胞测序是在单个细胞水平对基因组或转录组进行测序分析的技术,通过对单个细胞的DNA或RNA进行测序,表明组织系统层面的功能是由异质性细胞构成的,在解决生物异质性和生物材料的低获取量等问题上具有独特优势,极大改变了人们对许多生物现象的理解。本综述概括了当前单细胞测序技术发展、测序原理、测序流程以及在作物研究中的应用,并对当前已经取得成果的应用领域进行了阐述,以期为作物单细胞测序的研究与应用提供参考。

Individual cells of the same phenotype are commonly viewed as identical functional units of a tissue.The analysis of traditional detection method always bases on the overall average reaction of cells.Single-cell sequencing techniques are used in amplification and sequencing of the single-cell genome or transcriptome,the sequencing of DNA or RNA from single cells suggests the system-level function of a tissue is produced by heterogeneous cell.This technique presents numerous advantages for solving the problems of biological heterogeneity and low quantities of available biological materials.Here,we have summarized the the development of single-cell sequencing technology,the principle and applications in crops.We also introduce the successful applications in different fields,andwewish that this paperwould provides references of single cell sequencing study and application.