低氧预适应对氧糖剥夺/复糖复氧损伤的BV2细胞的保护作用及对其NLRP3表达的影响

Protective function of hypoxia preconditioning on oxygen-glucose deprivation/reoxygenation-induced BV2 cells injury and its effects on NLRP3 expression in BV2 cells

目的研究低氧预适应(HPC)对氧糖剥夺/复糖复氧(OGD/R)损伤的BV2细胞的保护作用及对其NLRP3表达的影响。方法将BV2细胞分为4组,分别为常氧组,HPC组,HPC+OGD/R组和OGD/R组,用MTS法测定细胞活力,蛋白免疫印迹测定BV2细胞中核苷酸结合寡聚化结构域样受体蛋白3(nucleotide-binding oligomerization domain-like receptor protein 3,NLRP3)蛋白的表达。结果与常氧组相比,OGD/R组的细胞活力明显降低(P<0.05)。给予1%O 2低氧2 h/复氧2 h的低氧预适应后,HPC+OGD/R组细胞活力明显高于其它HPC条件及OGD/R组的细胞活力(P<0.05),且HPC+OGD/R组NLRP3蛋白的表达显著低于OGD/R组(P<0.05)。结论低氧预适应可对氧糖剥夺/复糖复氧损伤的BV2细胞起到保护作用,其作用机制可能与其降低氧糖剥夺/复糖复氧损伤的BV2细胞NLRP3的表达水平有关。

Objective To study the protective effects of hypoxia preconditioning(HPC)on the injured BV2 cells induced by oxygen-glucose deprivation/reoxygenation(OGD/R)and its effects on nucleotide binding oligomerization domain-like receptor protein 3(NLRP3)expression.Methods BV2 cells were divided into 4 groups:normoxia group,HPC group,HPC+OGD/R group and OGD/R group,cell viability was determined by using MTS method,and the protein expression of NLRP3 in BV2 cells was determined by using Western Blot.Results Compared with the normoxia group,the cell viability of OGD/R group was significantly reduced(P<0.05).After receiving hypoxia preconditioning of 1%O 2 hypoxia for 2 hours/reoxygenation for 2 hours,the cell viability of HPC+OGD/R group was significantly higher than that of other HPC conditions and OGD/R group(P<0.05),and the protein expression of NLRP3 in HPC+OGD/R group was significantly lower than that in OGD/R group(P<0.05).Conclusion HPC may protect OGD/R-induced BV2 cells injury.The mechanism may be related to reducing NLRP3 protein expression in the injured BV2 cells induced by OGD/R.