核桃楸外果皮三萜类物质的提取及抑菌活性

Extraction of Triterpenoids from the Green Peel of Juglans mandshurica Maxim and its Antibacterial Activity

为探讨核桃楸外果皮三萜类物质提取方法的响应面优化,研究其抑菌活性,本文采用超声辅助的乙醇溶剂提取法提取核桃楸外果皮三萜类物质,并利用响应面法优化其提取工艺。在单因素实验的基础上,根据Box-Behnken中心组合法设计多因素试验,分析乙醇体积分数、超声时间和液料比对三萜类物质提取率的影响,确定最佳提取条件。此外,进一步研究三萜类物质对黄孢原毛平革菌(白腐真菌)和绿色木霉菌的抑菌活性。结果表明:核桃楸外果皮三萜类物质的最佳提取工艺条件为乙醇体积分数为80%、超声时间为44 min、液料比为19∶1(mL/g),此时核桃楸外果皮三萜类物质的提取率为2.62 mg/g。抑菌实验表明,核桃楸外果皮三萜类物质对黄孢原毛平革菌(白腐真菌)和绿色木霉菌均具有抑菌效果,最大抑菌圈直径分别达到18.43 mm和18.92 mm。本研究表明核桃楸外果皮三萜类提取物具有良好的抑菌活性,为核桃楸外果皮的开发和研究提供参考。

To optimize the extraction method of triterpenoids from the green peel of Juglans mandshurica Maxim by response surface, and to study its antibacterial activity, ultrasound-assisted ethanol extraction was used to extract the triterpenoids from the green peel of Juglans mandshurica, and the extraction process was optimized by response surface methodology(RSM). On the basis of single factor experiment, the optimal extraction conditions were explored through Box-Behnken central composite design, studying the effects of ethanol volume fraction, ultrasonic time and liquid-to-material ratio on the extraction rate of triterpenoids. In addition, the antibacterial activities of triterpenoids against Phanerochaete chrysosporium Burdsall and Trichoderma viride were further studied. The results showed that the optimal extraction conditions of triterpenoids from the green peel of Juglans mandshurica were: ethanol volume fraction 80%, ultrasonic time 44 min, liquid to material ratio 19∶1, and extraction rate was 2.62 mg/g. The bacteriostasis test showed that the triterpenoids of the green peel of Juglans mandshurica had antibacterial activities against Phanerochaete chrysosporium Burdsall and Trichoderma viride, the maximum inhibition zone diameter was 18.43 mm and 18.92 mm respectively. This study showed triterpenoids in the green peel of Juglans mandshurica had good bacteriostatic activity and provided a reference for the development and research of the green peel of Juglans mandshurica.