透明质酸/壳聚糖双层修饰的介孔生物活性玻璃的制备与性能研究

Preparation and Study of Hyaluronic acid/Chitosan Bilayered Mesoporous Bioactive Glass Scaffolds as Bioactive Drug Delivery System

本文制备介孔生物活性玻璃(MBG),进行氨基化修饰后,包裹透明质酸(HA)和壳聚糖(CS),制得透明质酸/壳聚糖双层修饰的介孔生物活性玻璃支架(MBG-HA/CS)。选择地塞米松(DEX)进行负载,研究药物释放情况。将成骨细胞(MC3T3)在MBG-HA/CS复合支架上进行培养,研究细胞的生长情况。通过扫描电镜和透射电镜观察,表明MBG-HA/CS具有孔径为100~300μm、相互连通的大孔结构和孔道约为3 nm的介孔结构。对于DEX的释放阶段,开始7 d以较快的速率进行释放,随后的21 d表现为稳定长期的释放过程。对培养在DEX@MBG-HA/CS支架上的MC3T3通过共聚焦显微镜进行观察,显示细胞生长良好。

In this study,mesoporous bioactive glass(MBG)was prepared and functionalized with amino groups.Then bilayer of hyaluronic acid/chitosan was assembled on mesoporous bioactive glass scaffolds(MBG-HA/CS).DEX was selected for loading and the DEX release in vitro from MBG-HA/CS was studied.Osteoblasts(MC3T3)were cultured on composite scaffolds to study the growth of osteoblasts.The results showed that MBG had a macroporous structure with 100~300μm interconnected pore size and a mesoporous structure with a pore size of about 3 nm as observed by scanning electron microscope and transmission electron microscope.During the release period,DEX was released from MBG-HA/CS at a fast rate in the first 7 days,and it can be released in a sustained and prolonged manner in the next 21 days.MC3T3 cultured on DEX@MBG-HA/CS scaffold were observed under confocal microscope,which showed the cells grew well.