连续股神经阻滞对KOA模型的疼痛治疗效果及可能的作用机制

The Effect of Continuous Femoral Nerve Block on Pain Treatment of KOA Model and Its Possible Mechanism of Action

目的:探讨连续股神经阻滞对兔KOA模型的疼痛治疗效果及可能的作用机制。方法:选择35只健康日本大耳白兔,建立KOA模型。将其随机分为7组:空白组、模型组及实验组(实验1组、2组、3组、4组及5组),予以连续股神经阻滞,观察7组疼痛反应、炎症反应表达。结果:与空白组相比,模型组疼痛评分明显增加(P<0.05);与模型组比较,实验组连续股神经阻滞时间越长,疼痛评分越低(P<0.05)。模型组IL-1、IL-6及TNF-α表达均高于空白组(P<0.05);与模型组相比,实验组IL-1、IL-6及TNF-α表达明显下降,其中实验3组、4组及5组下降明显(P<0.05)。与空白组比较,模型组、实验组血清P物质阳性表达明显增加,差异有统计学意义(P<0.05)。实验1~5组血清P物质阳性表达低于模型组,差异有统计学意义(P<0.05)。结论:连续股神经阻滞可减轻兔KOA疼痛程度,降低滑膜液内炎症介质表达。

Objective:To explore the effect and mechanism of continuous femoral nerve block on pain treatment of rabbit(KOA)model.ethod:35 healthy Japanese large-eared white rabbits were selected to establish a KOA process model.They were randomly divided into 7 groups:blank group,model group,and experimental group(Experimental Group 1,2,3,4,and 5 groups),and continuous femoral nerve block was given to observe the pain and inflammation expression in 7 groups.Result:Compared with the blank group,the pain score of the model group increased significantly(P<0.05);compared with the model group,the longer the continuous femoral nerve block time in the experimental group,the lower the pain score(P<0.05).The expressions of IL-1,IL-6 and TNF-αin the model group were higher than those in the blank group(P<0.05).Compared with the model group,the expressions of IL-1,IL-6 and TNF-αin the experimental group were significantly reduced.The decrease was significant in groups 3,4 and 5(P<0.05).Compared with the blank group,the positive expression of substance P in the serum of the model group and the experimental group increased significantly,and the difference was statistically significant(P<0.05).The positive expression of substance P in serum of group 1,2,3,4 and 5 of the experiment was lower than that of the model group,and the difference was statistically significant(P<0.05).Conclusion:Continuous femoral nerve block can reduce the degree of KOA pain and reduce the expression of inflammatory mediators in synovial fluid.