穿心莲有效成分的提取与分离研究

Separation of Active Ingredients from the Leaves of Andrographis Paniculata

探讨穿心莲有效成分的提取、分离方法。用95%乙醇冷浸提取穿心莲总内酯,然后用薄层层析的方法找出最佳洗脱剂,并在硅胶柱色谱上对提取物进行洗脱分离。结果发现,洗脱、分离穿心莲有效成分较好的洗脱剂是乙酸乙酯和比例为8∶1的氯仿、甲醇溶液;用8∶1的氯仿、甲醇溶液作洗脱剂,从提取物中分离出了5种物质,再用乙酸乙酯继续洗脱,分离出单个组分。

To investigate the extraction of active ingredients isolated Andrographis paniculata by the most simple,economical way.Method:Use 90%~95%ethanol solution of total lactones andrographolide extraction,and then use the method of TLCS to identify the best eluant,the eluant used in the column chromatography of silica gel extraction Separation of materials for elution.Results:The best eluant for Separation of andrographolide is ethyl acetate and the eight to one ratio of the chloroform methanol solution.Using 8∶1 chloroform and methanol solution as eluent,5 substances were separated from the extract,and then continued elution with ethyl acetate to separate a single component.