针吸细胞学联合qPCR技术诊断肺外结核病的价值

Diagnostic value of extra-pulmonary tuberculosis by fine needle aspiration cytology combined with qPCR for extrapulmonary tuberculosis

目的:探讨针吸细胞学(FNAC)联合荧光定量聚合酶链式反应(qPCR)对肺外结核病的诊断价值。方法:收集经FNAC判断为可能感染结核分枝杆菌或需排除感染结核分枝杆菌的57例患者的体表囊肿穿刺液。提取穿刺液中的DNA,并采用qPCR法检测其中有无结核分枝杆菌DNA。将qPCR检测结果、FNAC诊断与临床诊断进行比较。结果:临床确诊者23例,FNAC诊断符合率为82.61%(19/23),qPCR检测结果符合率为86.96%(20/23),2种方法联合应用可使诊断符合率达到92.98%(22/23)。结论:采用FNAC联合qPCR方法能对肺外结核病作出较准确诊断,准确率高于2种方法单独应用。

Objective:To explore the diagnostic value of fine needle aspiration cytology(FNAC)combined with fluorescence quantitative polymerase chain reaction(qPCR)for suspected extrapulmonary tuberculosis.Methods:A total of 57 patients probably infected by mycobacterium tuberculosis(TB)or needed to be excluded from infected TB were selected,and their puncture fluid of body surface cysts were collected.DNA was extracted from the puncture fluid and the qPCR method was used to detect the DNA of TB.The results of qPCR detection,FNAC,and clinical diagnosis were compared.Results:Based on clinical diagnosis(N=23),the diagnostic coincidence rate of FNAC was 82.61%(19/23),and the coincidence rate of qPCR test was 86.96%(20/23).The diagnostic coincidence of the combination of two methods was 92.98%(22/23).Conclusions:Fine needle aspiration cytology combined with qPCR can effectively diagnose the extrapulmonary tuberculosis,and the diagnosis rate is higher than single method.