摘要
建立博落回药材中四种生物碱的薄层色谱(TLC)鉴别方法。开展利用二甲苯-甲醇(5∶1)展开剂分离鉴别博落回中的原阿片碱、别隐品碱、血根碱和白屈菜红碱;利用氯仿-二甲苯-甲醇-氨水(50∶10∶1∶0.1)展开剂分离鉴别博落回中血根碱和白屈菜红碱;利用环己烷-丙酮(3∶2)展开剂分离鉴别博落回中原阿片碱和别隐品碱的系统研究。结果表明建立的薄层方法简便,准确和稳定,可以用于控制博落回药材的质量。
Established a thin-layer chromatography(TLC)method for the identification of 4 alkaloids in Macleaya cordata and M.microcarpa.To separate and identificate Protopine,Allocryptopine,Sanguinarine and Chelerythrine using xylene-methanol(5∶1)as the developer;To separate and identificate Sanguinarine and Chelerythrine using chloroform-xylene-methanol-ammonia(50∶10∶1∶0.1)as the developer;To separate and identificate Protopine and Allocryptopine using cyclohexane-acetone(3∶2)as the developer.The results showed that the established method is simple,accurate and stable,and can be used to control the quality of the medicinal materials.
作者
陈燕乐
杨广民
唐昭山
曾建国
CHEN Yan-le;YANG Guang-min;TANG Zhao-shan;ZENG Jian-guo(Hunan Micolta Biresource Co.,Ltd.,Changsha 410331,China;Hunan Engineering Research Center of Chinese Materia Medica Extracts,Changsha 410329,China;Hunan Key Laboratory of Veterinary Drugs,Hunan Agricultural University,Changsha 410128,China;National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative,Changsha 410128,China)
出处
《中国兽药杂志》
2020年第4期30-35,共6页
Chinese Journal of Veterinary Drug
基金
国家重点研发计划专项(2017YFD0501504)。
