IL-23/Th17相关细胞因子在基底细胞癌中的表达水平及意义

Expression and Significance of IL-23/Th17-related Cytokines in Basal Cell Carcinoma

目的探讨IL-23/Th17相关细胞因子在基底细胞癌(BCC)疾病进展中的作用。方法采用免疫组织化学染色的方法检测35例BCC组织及10例正常皮肤组织中IL-17、IL-22及IL-23的表达水平。结果IL-17在基底细胞癌中的表达水平高于正常皮肤组织(P<0.05),IL-17在基底细胞癌组织细胞和肿瘤间质细胞胞质中均有表达。IL-23在基底细胞癌中的表达水平高于正常皮肤组织(P<0.05),IL-23主要在基底细胞癌组织细胞胞质表达,部分位于肿瘤间质细胞胞质中。IL-22在基底细胞癌和正常皮肤组织的表达水平差异无统计学意义(P>0.05)。基底细胞癌中IL-17和IL-23的表达水平呈正相关(P<0.05)。结论IL-23/Th17相关细胞因子可能参与基底细胞癌的发生、发展。IL-23可能通过诱导Th17细胞的发育和增殖,促进IL-17分泌并在基底细胞癌的发生、发展中发挥协同作用。

Objective To investigate the role of IL-17/Th17-associated cytokines in the progression of basal cell carcinoma by measuring the expression of IL-17,IL-22 and IL-23 in basal cell carcinoma(BCC)and normal skin tissues.Methods Immunohistochemical staining was used to detect the expression levels of IL-17,IL-22 and IL-23 in 35 cases of BCC and 10 cases of normal skin.Results The expression level of IL-17 in basal cell carcinoma was higher than that in normal skin tissues(P<0.05).IL-17 was expressed in basal cell carcinoma tissue cells and tumor stromal cells.The expression level of IL-23 in basal cell carcinoma was higher than that in normal skin tissues(P<0.05).IL-23 was mainly expressed in the cytoplasm of BCC cells,and partly in the cytoplasm of tumor stromal cells.There was no significant difference in the expression levels of IL-22 between basal cell carcinoma and normal skin tissues(P>0.05).There was a positive correlation between the expression levels of IL-17 and IL-23 in basal cell carcinoma(P<0.05).Conclusion IL-23/Th17-related cytokines may be involved in the occurrence and development of basal cell carcinoma.IL-23 may promote the secretion and proliferation of Th17 cells,promote IL-17 secretion and play a synergistic role in the occurrence and development of basal cell carcinoma.