结肠癌组织中microRNA-340与ROCK1的表达及其临床意义

Expression and clinical significance of microRNA-340 and ROCK1 in tissues of colon cancer

目的:探讨microRNA-340(miR-340)与rho相关卷曲螺形成蛋白激酶1(rho associated-coi ledcoi l forming protein kinase,ROCK1)在结肠癌组织中的表达及二者的相互关系。方法:采用实时荧光定量PCR(real-time quantitative polymerase chain reaction,RT-qPCR)检测41例结肠癌组织及其癌旁正常黏膜组织中miR-340及ROCK1 mRNA的表达水平,同时采用蛋白质印迹法检测组织RohA/ROCK通路的表达,分析miR-340及ROCK1与大肠癌患者临床病理参数之间的关系。结果:结肠癌组织miR-340的相对表达量为0.757±0.056,低于癌旁正常黏膜组织的1.290±0.082,差异具有统计学意义(P<0.05),结肠癌组织ROCK1的相对表达量为1.551±0.078,高于癌旁正常黏膜组织的0.823±0.059,差异具有统计学意义(P<0.05)。结直肠癌组织的ROCK1,ROCK2及RhoA表达水平分别为0.455±0.038,0.385±0.033,0.852±0.075,正常黏膜组织分别为0.185±0.021,0.102±0.011,0.401±0.044,癌组织均高于正常组织,差异有统计学意义(P<0.05)。结肠癌组织中miR-340和ROCK1的表达与性别、年龄、发病部位、浸润深度、肿瘤分化程度无关(P>0.05),而与淋巴结转移及TNM分期有关(P<0.05)。Pearson相关分析结果示:结肠癌组织中miR-340与ROCK1表达呈负相关(r=?0.509,P<0.001)。结论:miR-340在结肠癌组织中表达下调,ROCK1表达上调,二者与淋巴结转移及TNM分期密切相关。miR-340可能通过抑制ROCK1表达发挥抑癌作用。

Objective: To explore the expression of microRNA-340 and rho associated-coiledcoil forming protein kinase(ROCK1) in tissues of colon cancer and its clinical significance. Methods: A total of 41 tissue specimens of colon cancer and adjacent normal tissues were chosen clinically. Quantitative real-time PCR were used to detect the different expression of miR-340 and ROCK1 mRNA in tissues. Western bloting assay was used to detect the expression of RohA/ROCK pathway. Results: miR-340 mRNA decreased significantly in colon cancer tissue(0.757±0.056) compared with the adjacent normal tissue(1.290±0.082), and the difference had statistical significance(P<0.05). ROCK1 mRNA increased significantly in colon cancer tissue(1.551±0.078) compared with the adjacent normal tissue(0.823±0.059), and the difference had statistical significance(P<0.05). Th e expression levels of ROCK1, ROCK2 and RhoA in colorectal cancer tissues were 0.455±0.038, 0.385±0.033 and 0.852±0.075, and the normal mucosal tissues were 0.185±0.021, 0.102±0.011 and 0.401±0.044, respectively. Those in the cancer tissues were higher than normal tissues, with statistically significant differences(P<0.05). Th e expression levels of miR-340 and ROCK1 were not associated with gender, age, locations, depth of tumor invasion and tumor differentiation(P>0.05). However, the expression levels of miR-340 and ROCK1 were associated with TNM stage and lymph node metastasis(P<0.05). Pearson correlation analysis revealed that the expression of miR-340 had a close negative correlation with ROCK1 in colon cancer(r=-0.509, P<0.001). Conclusion: Th e low expression of mi R-340 and high expression of ROCK1 are closed associated with TNM stage and lymph node metastasis. miR-340 may act as oncogene via targeting ROCK1 in colon cancer.