葛根素对人肝癌细胞HepG2侵袭与迁移能力的影响

Effects of puerarin on invasion and migration of human hepatocellular carcinoma HepG2 cells

目的探索葛根素对人肝癌细胞HepG2侵袭与迁移能力的影响及其相关分子机制。方法将人肝癌细胞HepG2分为两组,实验组用60μg/ml、30μg/ml的葛根素进行培养,对照组用加入等体积的葛根素溶解剂二甲基亚砜(DMSO)的培养基培养;利用细胞划痕实验检测两组细胞的迁移能力;利用Transwell小室检测两组细胞的侵袭能力;通过蛋白免疫印迹检测波形蛋白(vimentin)、E-钙黏蛋白(E-cadherin)、基质金属蛋白酶2/9(matrix metalloproteinase2/9,MMP2/9)。结果人肝癌细胞HepG2在经过葛根素培养后,细胞划痕的愈合能力下降,Transwell小室细胞的侵袭能力下降;E-cadherin表达上升(P<0.05),vimentin、MMP2和MMP9的表达下调(P<0.05)。结论葛根素通过上皮细胞间质转化(EMT)途径导致人肝癌细胞HepG2侵袭转移能力下降。

Objective To explore the effects of puerarin on the invasion and migration of human hepatocellular carcinoma HepG2 cells and their related molecular mechanisms.Methods The human hepatocellular carcinoma HepG2 cells were divided into two groups:experimental group and control group.The experimental group was cultured with puerarin of 60μg/ml and 30μg/ml,while the control group was cultured with medium added with equal volume of dimethyl sulfoxide(DMSO),a puerarin dissolving agent.The migration ability of the cells was detected in the two groups by cell scratch test,and the invasive ability was detected in the two groups by Transwell chamber.vimentin,epithelial cadherin(E-cadherin),matrix metalloproteinase 2/9(MMP2/9)were detected by Western blotting.Results After the human hepatocellular carcinoma HepG2 cells were cultured with puerarin,the healing ability of cell scratches decreased;the invasive ability of Transwell chamber cells decreased;the expression of E-cadherin(P<0.05)increased;the expression of vimentin decreased(P<0.05);and the expressions of MMP2 and MMP9 were down-regulated(P<0.05).Conclusion Puerarin reduces the invasion and metastasis of human hepatocellular carcinoma HepG2 cells by epithelial-mesenchymal transition(EMT).