miR-212在宫颈癌中的表达及对癌细胞增殖和侵袭的影响与机制

Expression of microRNA-212 in cervical cancer and its effect on proliferation and invasion of cancer cells

目的探讨miR-212在宫颈癌中的表达及其对宫颈癌细胞增殖和侵袭的影响及作用机制。方法采用RT-PCR和Western Blot方法检测宫颈癌细胞中miR-212和TCF7L2的表达水平。应用BrdU细胞增殖检测和Transwell侵袭实验分别检测细胞增殖和侵袭情况。运用生物信息学方法Target Scan预测并筛选miR-212的蛋白编码基因靶点,并应用双荧光素酶报告系统进行验证。结果与癌旁组织相比,miR-212在宫颈癌组织中的表达下调(P<0.01);与End1/E6E7细胞相比,miR-212在宫颈癌细胞系中的表达水平均显著降低(P<0.01)。miR-212的过表达可以抑制细胞的增殖和侵袭,而低表达miR-212可以促进宫颈癌细胞系的增殖和侵袭(P<0.01)。miR-212的过表达可以显著抑制TCF7L2蛋白的表达,而miR-212的低表达则促进TCF7L2蛋白的表达。靶基因TCF7L2是miR-212的直接作用靶点。结论宫颈癌组织中miR-212表达降低,通过基因TCF7L2的靶向调节抑制宫颈癌的侵袭和转移。

Objective To investigate the expression of miR-212 in cervical cancer and its effect on proliferation and invasion of cervical cancer cells and its mechanism.Methods RT-PCR and Western Blot were used To detect the expression levels of miR-212 and TCF7L2 in cervical cancer cells.BrdU cell proliferation assay and Transwell invasion assay were used to detect cell proliferation and invasion.Target Scan was used to predict the protein-coding gene target of miR-212,and double luciferase reporting system was used to validate it.Results The expression of mir-212 in cervical cancer tissues was down-regulated,compared with that in adjacent tissues(P<0.01).Compared with End1/E6E7 cells,the expression level of mir-212 in cervical cancer cell lines was significantly reduced(P<0.01).Overexpression of miR-212 can inhibit cell proliferation and invasion,while low expression of miR-212 can promote the proliferation and invasion of cervical cancer cell lines(P<0.01).Overexpression of miR-212 can significantly inhibit the expression of TCF7L2 protein,while low expression of miR-212 can promote the expression of TCF7L2 protein.The target gene TCF7L2 is the direct target of miR-212.Conclusion The expression of miR-212 in cervical cancer tissue was decreased,and the invasion and metastasis of cervical cancer were inhibited by targeting regulation of TCF7L2 gene.