sclerostin在大鼠实验性牙周炎牙槽骨中的表达

Expression of sclerostin in alveolar bone of experimental periodontitis in rats

目的观察大鼠实验性牙周炎牙槽骨中sclerostin的表达与分布。方法选用16只3月龄左右的SD大鼠,随机分为4组(1个空白对照组和3个实验组),实验组利用丝线结扎上颌左右第二磨牙制作牙周炎模型,各组分别于0、3、7、14 d处死大鼠,取实验部位组织,用Masson染色检验牙周炎建模成功与否,免疫组织化学的方法检测sclerostin的表达与分布。结果丝线结扎牙颈部制作大鼠牙周炎模型,方法成熟可靠。sclerostin在正常成年大鼠牙槽骨组织中偶见表达;3 d组sclerostin染色阳性骨细胞数成倍增加,7 d组达到高峰,14 d组表达开始下降。结论细胞通过合成sclerostin参与牙周炎的发病与发展过程;牙槽骨的破坏与改建,表达随时间呈现一定规律。

Objective To observe the expression and distribution of sclerostin in alveolar bone of experimental periodontitis in rats.Methods 16 SD rats of about 3 months old were randomly divided into 4 groups(1 blank control group and 3 experimental groups).The rats were sacrificed at 0,3,7,and 14 d,and the experimental tissue was taken.The expression and distribution of sclerostin were detected by immunohistochemistry.Results The model of rat periodontitis is made by ligating the teeth with silk,and the method is mature and reliable.Sclerostin was occasionally expressed in alveolar bone tissue of normal adult rats.On the third day,the number of sclerostin-positive bone cells increased exponentially,the 7 d group reached a peak,and the 14 d group began to decrease.Conclusion The cells participate in the pathogenesis and development of periodontitis through the synthesis of sclerostin;the destruction and reconstruction of alveolar bone,the expression shows a certain regularity with time.