沉默FGL1增强人肺腺癌PC-9细胞对多西他赛敏感性的研究

Enhancive effect of FGL1 gene silencingon sensitivity of human lung adenocarcinoma PC-9 cells to docetaxel

目的探讨沉默纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)对人肺腺癌PC-9细胞多西他赛敏感性的影响。方法Western blot法检测人正常支气管上皮细胞株BEAS-2B及人肺腺癌细胞株PC-9中的FGL1蛋白表达情况。以siRNA沉默PC-9细胞株中FGL1基因,CCK-8法检测沉默FGL1对PC-9细胞增殖的抑制作用及对多西他赛敏感性的影响。结果与BEAS-2B细胞株比较,FGL1在PC9细胞株中呈高表达,其蛋白相对表达量是BEAS-2B细胞株的6.5倍,差异具有显著性(P<0.01)。通过转染FGL1siRNA沉默FGL1可增强多西他赛对PC-9细胞增殖的抑制作用。与FGL1siNC组细胞相比,FGL1siRNA组PC-9细胞的IC50值明显降低,差异具有显著性(P<0.01)。结论特异性沉默FGL1基因可抑制人肺腺癌细胞PC-9中FGL1的表达,抑制PC-9细胞的增殖,提高对多西他赛的敏感性。

Aim To investigate the effect of fibrinogen-like protein 1(FGL1)silencing on docetaxel sensitivity in human lung adenocarcinoma PC-9 cells.Methods Western blot was used to detect the expression of FGL1 protein in human normal bronchial epithelial cell line BEAS-2B and human lung adenocarcinoma cell line PC-9.The FGL1 gene in PC-9 cell line was silenced by siRNA.CCK-8 assay was used to detect the inhibitory effect of silencing FGL1 on PC-9 cell proliferation and its effect on docetaxel sensitivity.Results Compared with BEAS-2B cell line,FGL1 was highly expressed in PC-9 cell line,and the relative expression of FGL1 protein was 6.5 times that of BEAS-2B cell line with statistically significant difference(P<0.01).Silencing FGL1 by transfection with FGL1siRNA could enhance the inhibitory effect of docetaxel on PC-9 cells.Compared with FGL1siNC group,the IC50 value of PC-9 cells in FGL1siRNA group was significantly reduced with statistically significant difference(P<0.01).Conclusions Specific silencing of FGL1 gene could inhibit the expression of FGL1 in human lung adenocarcinoma cell PC-9,inhibit the proliferation of PC-9 cells and increase the sensitivity to docetaxel.