五味子丙素缓解高脂饮食喂养ApoE-/-小鼠的动脉粥样硬化与炎症

Schisandrin C alleviates atherosclerosis and inflammation in ApoE-/-mice fed with arterial high-fat diet

目的探究五味子丙素(五味子醇C,schisandrin C,Sch C)对高脂饮食喂养ApoE-/-小鼠的动脉粥样硬化和炎症的影响。方法将ApoE-/-小鼠分为高脂饮食喂养组(HFD)、高脂饮食喂养+五味子丙素组(HFD+Sch C),高脂饮食和药物同时给予,12周后,处死小鼠,收集动脉组织。采用RT-qPCR法检测IL-6、TNF-α、ICAM-1和β-actin的mRNA表达;油红O染色检测主动脉根部油脂变化;生化试剂盒检测血液中的血脂水平。Western blot法检测动脉的IκB-α磷酸化与表达。结果与HFD组比较,五味子丙素治疗组动脉粥样硬化斑块面积减少(P <0. 05),但五味子丙素不影响血脂水平;与HFD组比较,五味子丙素治疗组动脉中TNF-α、IL-6和ICAM-1的mRNA表达水平和IκB-α磷酸化均显著降低(P <0. 05)。结论五味子丙素能有效缓解高脂饮食喂养诱导的小鼠动脉粥样硬化,并伴随缓解动脉炎症反应。

Aim To investigate the effects of schisandrin C on arterial inflammation and atherosclerosis of ApoE^-/-mice fed with high-fat diet.Methods ApoE^-/-mice were divided into high fat diet group(HFD)and high fat diet group+schisandrin group(HFD+Sch C).High fat diet and Sch C were given at the same time.After 12 weeks,mice were executed and arterial tissues were collected.RT-q PCR and Western blot were respectively used to detect the expressions of IL-6,TNF-α,ICAM-1,β-actin and the phosphorylation and expression of IκB-αin arteries.Oil red O staining and biochemical kit were respectively used to detect the lipid changes in aortic root and the blood lipid levels.Results Compared with HFD group,the area of atherosclerotic plaque in HFD+Sch C was reduced(P<0.05),but Sch C did not affect blood lipid levels.Compared with HFD group,the mRNA expression of TNF-α,IL-6 and ICAM-1 and the phosphorylation of IκB-αof arterial tissue in HFD+Sch Cgroup decreased(P<0.05).Conclusions Sch C could effectively alleviate atherosclerosis induced by high-fat diet in ApoE^-/-mice,accompanied by alleviation of arterial inflammation.