Runt相关转录因子2蛋白表达与肺腺癌侵袭转移的关系

Correlation between the expressions of runt-related transcription factor 2 protein and aggressiveness and metastasis of lung adenocarcinoma

目的探讨肺腺癌组织中Runt相关转录因子2(Runx2)蛋白的表达与肿瘤侵袭转移的关系。方法收集49例肺腺癌患者的临床资料及病理标本(包括肺腺癌组织、癌旁组织及正常肺组织)。应用免疫组织化学和Western blotting方法检测各相关组织中Runx2蛋白的表达,对Runx2蛋白表达与临床病理参数的关系进行统计学分析。结果Runx2蛋白在肺腺癌组织、癌旁组织、正常肺组织中的阳性表达率分别为57.1%、8.2%、0,差异具有统计学意义(P<0.001);Runx2蛋白高表达与浸润性腺癌(26/34,76.47%)、有淋巴结转移(13/15,86.67%)、低分化(14/14,100%)、随访期内出现复发转移(8/8,100%)显著相关(P<0.001,P=0.006,P=0.006,P=0.007)。结论肺腺癌组织中Runx2蛋白高表达,且与肿瘤的低分化、高侵袭转移特性及复发的关系密切。

Objective To explore the expression of runt-related transcription factor 2(Runx2)protein in lung adenocarcinoma and to investigate their relationship with aggressiveness and metastasis.Methods Clinical data and pathological specimens of 49 patients with lung adenocarcinoma(including lung adenocarcinoma tissues,paracancer tissues and normal lung tissues)were collected to detect the expressions of the Runx2 by immunohistochemical and Western blotting method.The correlation between the expressions of Runx2 protein and aggressiveness and metastasis were analyzed by SPSS software.Results The positive expression rates of Runx2 protein in lung adenocarcinoma,paracancer and normal lung tissues were 57.1%,8.2%and 0,respectively,with statistically significant differences(P<0.001).The high expression of Runx2 protein was closely related to invasive adenocarcinoma(26/34,76.47%),lymphnode metastasis(13/15,86.67%),poorly differentiated(14/14,100%),recurrence and metastasis during follow-up(8/8,100%),and the relationship was significant(P<0.001,P=0.006,P=0.006,P=0.007,respectively).Conclusions The high expression of Runx2 protein in lung adenocarcinoma tissues is closely related to the low differentiation,high invasion and metastasis characteristics and recurrence of the tumor.