消风宣窍汤对OVA诱导的变应性鼻炎小鼠脾脏代谢特征的作用研究

Effect of Xiaofeng XuanQiao Decoction on the Characteristics of Spleen Metabolism in OVA-Induced Allergic Rhinitis Mice

目的通过对变应性鼻炎(AR)模型小鼠脾脏代谢谱变化的分析,探讨消风宣窍汤对AR的治疗效应和可能机制。方法将60只Balb/c小鼠随机分为对照组、模型组、孟鲁司特钠组、消风宣窍汤组,采用卵白蛋白(OVA)致敏建立小鼠AR模型,将各组脾脏取出,经甲醇提取,肟化和衍生化后,利用GC-MS分析消风宣窍汤干预后对AR小鼠脾脏样本代谢谱的影响。结果AR小鼠的脾脏代谢谱发生显著变化,共鉴定出45种代谢产物发生显著变化,主要涉及苯丙氨酸、乳酸、亮氨酸、β-丙氨酸、苯甲醇、3-羟基丁酸等;消风宣窍汤干预后能够逆转苯甲酸、乙醇胺、甘油-1-磷酸、3-羟基丁酸、苯甲醇、β-丙氨酸、肌苷、天冬氨酸、乳酸、苯丙氨酸、甲醇磷酸盐、O-磷酸乙醇胺、烟酰胺、焦磷酸,而孟鲁司特钠无明显回归趋势。结论AR小鼠脾脏中多种代谢物成分发生变化,消风宣窍汤可通过调控苯丙氨酸、酪氨酸和色氨酸的生物合成,β-丙氨酸代谢相关的脾脏代谢物,使其趋于回归,从而发挥治疗AR的作用。

OBJECTIVE To analyze the changes of spleen metabolic profile in mice with allergic rhinitis(AR)and the therapeutic effect and possible mechanism of Xiaofeng Xuanqiao Decoction on AR.METHEODS 60 BALB/c mice were randomly divided into control group,model group,montelukast sodium group and Xiaofeng Xuanqiao Decoction group.The AR model was established by OVA sensitization.The spleen of each group was taken out through methanol.After extraction,methoxypyridine and BSTFA were successively deuterated and derivatized.The effect of Xiaofeng Xuanqiao Decoction on the metabolic profile of AR spleen samples was analyzed by GC-MS.RESULTS The spleen metabolic profile of AR mice changed significantly.45 kinds of metabolites were identified,mainly including pyruvic acid,lactic acid,leucine,β-alanine,benzyl alcohol,3-hydroxybutyric acid.Xiaofeng Xuanqiao Decoction reversed 3-hydroxybutyric acid,benzoic acid,benzyl alcohol,β-alanine,ethanolamine,glycerol-1-phosphate,inosine,aspartic acid,lactic acid,phenylalanine,methanolphosphate,niacinamide,O-phosphoethanolamine,pyrophosphate,while montelukast sodium group showed no obvious return trend.CONCLUSION Xiaofeng Xuanqiao Decoction can regulate the biosynthesis of phenylalanine,tyrosine and tryptophan andβ-alanine metabolism,and play a role in the treatment of AR.