烟草CYP71D亚家族的生物信息学分析

Bioinformatic Analysis of CYP71D Subfamily Genes in Tobacco(Nicotiana tabacum L.)

细胞色素P450(CYP450)是一类超基因家族编码的多功能氧化酶,在生物合成、代谢解毒及植物防御方面具有重要作用。其中CYP71D属于CYP450的一个亚家族,主要在次生代谢物合成和病虫害防御方面起作用。为了更好地了解烟草CYP71D亚家族基因特征和功能,本研究利用生物信息学分析手段及烟草转录组数据,对CYP71D亚家族基因的结构和表达等进行了分析。结果发现,在烟草中共有42个CYP71D亚家族成员,各成员在染色体上并非均匀分布,其氨基酸长度和等电点差异较大,但基因结构、保守结构域数目和分布高度一致;二级结构分析表明,烟草CYP71D亚家族成员具备P450蛋白特征结构域;基因表达模式分析显示,多数CYP71D基因在根、叶、花中特异表达,大部分基因参与了IAA激素、黑胫病、温度等逆境胁迫反应。这为烟草CYP71D亚家族基因功能的深入研究及烟草抗逆品种的培育奠定了基础。

Cytochrome P450(CYP450)is a supergene family which encodes multifunctional oxidase,playing an important role in biosynthesis,metabolic detoxification,and plant defense.In CYP450 family,the CYP71D subfamily genes mainly function in secondary metabolite synthesis and pest control.In order to better understand the genetic characteristics and functions of tobacco CYP71D subfamily genes,their gene structure and expression pattern were analyzed by bioinformatics tools and transcriptome data.The results showed that there are 42 members in tobacco CYP71D subfamily and they were distributed on the chromosome unevenly.Besides,their amino acid lengths and isoelectric points were quite different,while their gene structure and conserved-domain distribution were highly consistent.The secondary structure analysis showed that CYP71D subfamily members contained the typical conserved domains in P450 proteins.The gene expression pattern analysis showed that most CYP71D genes were specifically expressed in roots,leaves and flowers,and some of them were induced by various elicitors,including phytohormone IAA,temperature and black shank.This study contributes to further researches in the function of CYP71D subfamily genes and breeding for stressresistant tobacco cultivars.