过表达MdCBF1基因提高植物抗冷性

Overexpression of MdCBF1 Increases Cold Tolerance of Plant

本试验将从‘嘎啦’苹果中克隆的MdCBF1转化到‘王林’苹果愈伤组织和野生型拟南芥幼苗中,进行低温冷处理,观察并比较分析野生型与转MdCBF1材料在成活率和表达量间的差异,以研究MdCBF1基因对植株抗冷性的影响。结果显示,低温处理后,与野生型植株相比,转基因苹果愈伤组织抗冷性更高,转基因拟南芥成活率更高,抗冷性相关基因表达水平更高。可以看出,MdCBF1过表达能够显著提高植株的抗冷性,表明其在植物的冷胁迫响应过程中发挥着重要作用。

In order to explore the function of MdCBF1 in cold stress response at low temperature,we cloned the MdCBF1 gene from Gala apple(Malus×domestica)and transformed into Wanglin apple calli and wild Arabidopsis seedlings.After treated with low temperature,the changes in survival rate and gene expression levels were studied.The results showed that the cold tolerance of transgenic apple calli was higher than that of wild type;the survival rate and gene expression level of transgenic Arabidopsis seedlings were higher than those of wild type after low temperature treatment.In conclusion,the overexpression of Md CBF1 could significantly increase the cold tolerance of plants,which suggested that Md CBF1 play an important role in cold response of plant.