miR-222-3p靶向SOCS5促进宫颈癌细胞增殖、迁移和侵袭

miR-222-3p Promotes Proliferation, Migration and Invasion of Cervical Cancer Cells by Targeting SOCS5

目的明确miR-222-3p对宫颈癌细胞的生物学影响。方法采用qRT-PCR检测宫颈癌患者血清和宫颈癌细胞系miR-222-3p的表达水平,通过CCK-8检测miR-222-3p对宫颈癌细胞增殖能力的影响;进一步采用划痕实验和Transwell实验明确miR-222-3p对宫颈癌细胞迁移和侵袭能力的影响;利用在线数据库预测miR-22-3p的潜在靶点,并验证其对宫颈癌细胞恶性表型的影响。结果miR-222-3p在宫颈癌患者血清和宫颈癌细胞系SiHa中表达上调,miR-31-5p促进Siha细胞增殖、迁移和侵袭能力。荧光素酶报告基因实验证实SOCS5是miR-222-3p的直接靶点,SOCS5过表达质粒会部分抑制miR-222-3p对SiHa细胞的增殖、迁移和侵袭。结论上调表达的miR-222-3p通过抑制SOCS5增强宫颈癌细胞增殖、迁移和侵袭能力,可能参与宫颈癌的发病。

Objective To explore the biological effects of miR-222-3p on cervical cancer cells.Methods The expression of miR-222-3p was evaluated by qRT-PCR in patients with cervical cancer and cervical cancer cell line SiHa.CCK-8 assay was performed to determine the role of miR-222-3p in proliferation of SiHa cells.Next,we carried out wound healing assay and Transwell assay to detect the effect of miR-222-3p on migration and invasion of SiHa cells.The potential target of miR-222-3p was predicted by TargetScanHuman database,and verified by luciferase reporter assay.Finally,the malignant phenotypes of the target of miR-222-3p were evaluated.Results The expression of miR-222-3p was overexpressed in patients with cervical cancer and SiHa cells,and miR-222-3p promoted proliferation,migration and invasion of SiHa cells.Next,we identified SOCS5 as the direct target of miR-222-3p.Further investigation revealed that SOCS5 overexpression could partially inhibited proliferation,migration and invasion of SiHa cells induced by miR-222-3p.Conclusion The upregulated miR-31-5p facilitated proliferation,migration and invasion of cervical cancer cells by inhibiting SOCS5,which might contribute to pathogenesis of cervical cancer.