卷烟挥发性香气成分测定及烟丝加香工艺优化

Determination of volatile aroma components in cigarettes and optimization of flavoring process of tobacco

建立了一种顶空—搅拌棒固相微萃取—热脱附—气相色谱—质谱联用(HS-SBSE-TD-GC-MS)检测卷烟烟丝中11种挥发性物质的方法,将其应用于烟丝加香工艺中的参数优化,分别对样品预处理方式和热脱附操作条件进行了优化。结果表明,①在优化的试验条件下,相关系数R2>0.998,检出限和定量下限分别为0.052~0.524,0.172~1.684μg/g;②加标回收率在85.2%~97.6%,测定结果的相对标准偏差在2.7%~6.0%;③基于该方法研究优化了加香工艺滚筒频率和风门大小参数,得出与感官评吸中香气评定一致的最佳加香工艺参数为滚筒频率40r/min,风门大小90%。该方法灵敏度高、线性关系好,可以作为烟丝加香工艺参数优化的有效手段。

A method for the determination of 11 volatile substances in cigarette cut tobacco by headspace-stirring rod solid phase microextraction-thermal desorption-gas chromatography-mass spectrometry(HS-SBSE-TD-GC-MS)was established.The parameters were optimized in the tobacco flavoring process,and the sample pretreatment method and thermal desorption operation conditions were optimized.①Under the control of the optimized experimental conditions,the correlation coefficient R 2 was higher than 0.998,and the detection limit and the lower limit of quantitation were 0.052~0.524μg/g and 0.172~1.684μg/g,respectively;②The spike recovery was at 85.2%~97.6%and the relative standard deviation of the determination results was at 2.7%~6.0%;③Through this method,the flavoring process drum frequency and damper size were optimized as 40 r/min and 90%,respectively,and the best aroma evaluation in sensory evaluation results were obtained under the control of these conditions.The method had high sensitivity and good linearity,and could be used as an effective means to optimize the parameters of tobacco flavoring process.