松毛菇多酚抗氧化及抑菌活性研究

Antioxidant and antibacterial activity of polyphenols from Lyophyllum Cinoraseens Konr&Maubl

以超声波辅助乙醇提取松毛菇粗多酚和经大孔树脂纯化的松毛菇纯化多酚为原料,研究了松毛菇多酚对·OH、O_2^-·和DPPH·的清除作用,并应用滤纸片法研究其抑菌活性。结果表明,松毛菇粗多酚和纯化多酚均具有清除·OH、O_2^-·和DPPH·的作用,且清除能力均与多酚质量浓度呈量效关系。松毛菇粗多酚及纯化多酚对5种供试菌(大肠埃希氏菌、伤寒沙门氏菌、金黄色葡萄球菌、枯草芽孢杆菌、酿酒酵母)表现出不同的抑菌活性,相同浓度下两者对酿酒酵母的抑菌能力最强,纯化多酚对大肠埃希氏菌的抑菌能力最弱,粗多酚对大肠埃希氏菌无抑制作用。纯化多酚对5种供试菌的抑菌作用与其质量浓度呈正相关,除大肠埃希氏菌外,粗多酚对其他供试菌的抑菌作用亦与多酚浓度呈正相关。相同浓度下纯化多酚的抑菌活性强于粗多酚。

In order to investigate the antioxidation and antibacterial effect of polyphenols from Lyophyllum Cinoraseens Konr&Maubl,crude polyphenols after ultrasonic-assisted ethanol extraction purified polyphenols with macroporous resin were used as raw materials to study the scavenging effect for·OH,O-2·and DPPH·and antibacterial effect by the filter paper disc method.The results showed that crude polyphenols and purified polyphenols from Lyophyllum Cinoraseens Konr&Maubl both exhibit the scavenging effect for·OH,O-2·and DPPH·,and there presented certain dose-effect relationship between scavenging ability and the concentration of polyphenols.Crude polyphenols and purified polyphenols showed different antibacterial activity against five tested microorganisms(Escherichia coli,Salmonella typhi,Staphylococcus aureus,Bacillus subtilis,Saccharomyces cerevisiae),and both of them had the strongest antibacterial effect on Saccharomyces cerevisiae under the same concentration.Purified polyphenols had the weakest antibacterial effect on Escherichia coli,while crude polyphenols had no antibacterial effect on Escherichia coli.The inhibitory effect on five tested microorganisms was positively correlated with the mass concentration of purified polyphenols.In addition to Escherichia coli,the inhibitory effect of on other four tested microorganisms was also positively correlated with the concentration of crude polyphenols.The antibacterial activity of purified polyphenols was better than that of crude polyphenols at the same concentration.