余甘子叶总黄酮的超声波法提取工艺优化及其抗氧化能力研究

Optimization of ultrasonic-assisted extraction of total flavonoids from leaves of phyllanthus emblica and its antioxidant capacity

以余甘子叶为原料,采用超声法提取总黄酮物质,并进行抗氧化能力测定。结果表明,余甘子叶总黄酮的最佳提取工艺为料液比1:25(g/mL)、乙醇浓度60%、超声功率70 W、提取时间20min、提取温度60℃,此条件下的余甘子叶总黄酮提取率高达14.57%。余甘子叶总黄酮具有较强的自由基清除能力,当浓度为0.5mg/mL时,·OH清除率为82.72%,NaNO_2清除率为66.14%;当浓度为0.08mg/mL时,DPPH·清除率为97.40%。

Optimization ultrasonic-assisted extraction of total flavonoid from leaves of phyllanthus emblica was investigated and its antioxidant capacity was determined.Through single factor experiment and orthogonal experiment,the optimization of ultrasonic-assisted extraction of total flavonoids was determined as follows,including the ratio of material to liquid 125(g/mL),ethanol concentration 60%,ultrasonic power 70 W,and extraction at 60℃for 20 min.Under the control of these conditions,the extraction rate of the total flavonoids from leaves of P.emblica was as high as 14.57%.Moreover,the total flavonoids from leaves of P.emblica were found good scavenging effect.With the use of 0.5 mg/mL total flavonoid,the clearance rate of·OH was found to be 82.72%,and that of NaNO 2 was 66.14%.When 0.08 mg/mL total flavonoid was used,the clearance rate of DPPH·was determined to be 97.40%.