磷酸核糖焦磷酸合成酶2在人睾丸组织中的表达

Expression of phosphoribosyl pyrophosphate synthase 2 in human testis tissue

目的:探讨磷酸核糖焦磷酸合成酶2(PRPS2)在人睾丸组织中的表达及其临床意义. 方法:qRT-PCR定量检测PRPS2 mRNA在生精功能正常,轻度、中度及重度生精功能障碍睾丸组织中的表达;免疫组化检测PRPS2蛋白在67例成年男性睾丸活检组织中的表达,并探讨其表达与睾丸组织学类型及临床参数的关系. 结果:qRT-PCR检测结果显示,PRPS2 mRNA在生精功能正常睾丸组织中的表达水平明显高于中度及重度生精功能障碍睾丸组织(P<0.01);免疫组化检测结果显示,PRPS2阳性表达于生精功能正常及轻度生精功能障碍睾丸组织,而在中度及重度生精功能障碍睾丸组织中的表达明显减弱,其在生精功能正常、轻度生精功能障碍、中度生精功能障碍及重度生精功能障碍睾丸组织中的阳性表达率分别为70.0%、66.7%、50.0%、23.8%,差异具有统计学意义(P=0.031).PRPS2表达与临床参数未见明显相关(P>0.05). 结论:PRPS2低表达于生精功能障碍的睾丸组织,其表达水平的检测有助男性不育的诊断及睾丸生精功能的预测.

Objective:To investigate the expression of phosphoribosyl pyrophosphate synthase 2(PHPS2)in the human testis and its clinical significance.Methods:Using quantitative real-time PCR(qRT-PCR)and immunohistochemistry,we detected the expression of PRPS2 mRNA in the testis tissue of the men with normal spermatogenesis or mile,moderate or severe hypospemiatogenesis(HS)and that of the PRPS2 protein in the testicular biopsy tissue of 67 adult males.Then,we analyzed the relationship of the PRPS2 expressions with the testicular histological types and clinical parameters of the subjects.Results:The expression of PRPS2 m HNA in the testis tissue was significantly higher in the normal spermatogenesis group than in the moderate and severe HS groups(P<0.01).The positive expression of the PRPS2 protein was 70.0%in the normal spermatogenesis group,66.7%in the mild HS group,50.0%in the moderate HS group and 23.8%in the severe HS group,significantly higher in the normal spermatogenesis and mild HS groups than in the moderate and severe HS groups(P<0.01).No significant correlation,however,was observed between the PRPS2 expression and clinical parameters of the subjects(P>0.05).Conclusion:PRPS2 is lowly expressed in the testis tissue of the men with hypospermatogenesis and its expression level may help the diagnosis of male infertility and the prediction of the spermatogenic function of the testis.