艰难梭菌毒素B诱导结肠癌细胞凋亡及相关机制研究

Clostridium difficile toxin B-induced apoptosis of colon cancer cells and related mechanisms

目的检测艰难梭菌毒素B(TcdB)对结肠癌SW480细胞增殖与凋亡的影响,研究其引起细胞凋亡的相关机制。方法采用不同浓度的TcdB处理SW480细胞,采用MTT法检测细胞增殖情况;用流式细胞术检测细胞的凋亡及线粒体膜电位变化情况。结果TcdB显著抑制了结肠癌SW480细胞的增殖,作用48 h后的抑制率为46.36%,呈一定的时间-浓度相关性;流式细胞仪检测结果表明,浓度为800 ng/ml的TcdB作用48 h,SW480细胞凋亡率为20.83%,呈一定的时间-浓度相关性。结论艰难梭菌毒素B能够抑制结肠癌SW480细胞增殖、诱导细胞凋亡,其机制可能与启动线粒体凋亡途径有关。

Objective To analyze the effects of Clostridium difficile toxin B(TcdB)on the proliferation and apoptosis of colon cancer cell line SW480 and the possible mechanisms related to cell apoptosis.Methods SW480 cells were treated with different concentrations of TcdB.Cell proliferation was detected by MTT assay.Cell apoptosis and mitochondrial membrane potential were measured with flow cytometry.Results TcdB significantly inhibited the proliferation of SW480 cells in a time-concentration dependent manner and the inhibition rate reached 46.36%at 48 h.Flow cytometry results showed that TcdB could induce the apoptosis of SW480 cells in a time-concentration dependent manner and a 20.83%apoptosis rate was induced by 800 ng/ml of TcdB at 48 h.Conclusions TcdB could inhibit the proliferation and induce the apoptosis of colon cancer SW480 cells,and the possible mechanisms might be relate to the initiation of mitochondrial apoptosis pathway.