分子印迹固相萃取柱-HPLC测定植物油中苯并芘[a]的含量

Determination of Benzopyrene[a]in Vegetable Oil by Molecular Imprinting Column and HPLC

建立了一种植物油中苯并芘[a]经正己烷溶解,通过分子印迹柱吸附,二氯甲烷洗脱,经过C18的反相色谱柱分离的检测方法。流动相甲醇-水(88∶12,V/V);柱温35℃;进样体积20μL荧光检测器检测。发射波长384 nm,激发波长406 nm。经测试分析,该方法的最低检出限为0.10μg/kg。线性范围0.5-20 ng/mL。回收率95.6-102.3%。相对标准偏差在2.93-6.81%。该方法分析步骤简单,特异性强、回收率高,重复性好,测定结果准确,适合于植物中苯并芘[a]的测定。

A benzopyrene in vegetable oil[a]was dissolved by n-hexane.Through molecular imprinting column adsorption,Methylene dichloride elution and separated by C18 reverse chromatography column.Methanol-water in mobile phase(88∶12,V/V);Column temperature 35℃;The sample volume was detected by 20 micron fluorescence detector.The emission wavelength is 384 nm and the excitation wavelength is 406 nm.The minimum detection limit of the method is 0.10μg/kg.The linear range is 0.1 ng/mL 200 ng/mL.The recovery was 95.6-102.3%.The relative standard deviation is 2.93-6.81%.The method has the advantages of simple analysis procedure,high specificity,high recovery and good repeatability,suitable for the determination of benzopyrene[a]in plants.