藏红花改善酒精性肝病患者的氧化应激作用及对Caspase-3、Bcl-2水平的影响

Saffron adjuvant therapy improved oxidative stress in alcoholic liver disease patients and its effect on Caspase-3 and Bcl-2 levels

目的:探讨藏红花对酒精性肝病患者的疗效以及改善氧化应激的作用和对半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)和B细胞淋巴瘤/白血病-2(Bcl-2)水平的影响。方法:选取2014年1月至2018年12月就诊的酒精性肝病患者120例,按数字表法随机分为对照组和治疗组各60例。对照组患者依指南给予常规措施。治疗组患者在对照组基础上给予藏红花治疗,每次1 g,1次/d。连续治疗8周后,比较两组患者肝功能、临床疗效、氧化应激指标[血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)]水平以及血清Caspase-3和Bcl-2水平。结果:治疗后:治疗组患者肝功能指标天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆红素(TBil)、谷氨酰氨基转移酶(GGT)明显低于对照组(P<0.01)。治疗组患者总有效率(93.33%)显著高于对照组(79.66%)(P<0.05);治疗组患者血清SOD、GSH-PX水平显著高于对照组,MDA水平明显低于对照组(P<0.01);治疗组患者血清Caspase-3、Bcl-2水平显著少于对照组(P<0.01)。结论:在常规治疗基础上加用藏红花可有效改善酒精性肝病患者的肝功能,提高临床疗效,改善氧化应激水平以及下调Caspase-3、Bcl-2水平。

Objective:To investigate the efficacy of saffron adjuvant therapy on alcoholic liver disease and the improvement of oxidative stress and its effects on levels of cysteine aspartate protease-3(caspase-3)and B cell lymphoma-leukemia-2(bcl-2).Methods:One hundred-twenty alcoholic liver disease cases admitted to our hospital from January 2014 to December 2018 were selected and randomly divided into treatment group and control group(60 cases in each group).The control group was given with routine measures according to the guidelines.The treatment groupwas treated with saffron(1 g per day and qd)on the basis of the control group.After 8 weeks’continuous treatment,liver function,the efficacy,oxidative stress[serum superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA)]levels and serum caspase-3 and bcl-2 levels were compared between the two groups.Result:After 8 weeks’treatment,aspartate aminotransferase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL),and glutamine aminotransferase(GGT)were significantly lower in the treatment groupthan in the control group(P<0.01).The total effective rate(93.33%)in the treatment groupwas significantly higher than that in the control group(79.66%)(P<0.05).Serum levels of SOD and GSH-PX in the treatment groupwere significantly higher than those in the control group,while MDA levels were significantly lower than those in the control group(P<0.01).Serum caspase-3 and bcl-2 levels in the treatment group were significantly lower than the control group(P<0.01).Conclusion:On basis of routine therapy,Saffron can effectively improve the liver function of with alcoholic liver disease patients,increase the efficacy,improve the level of oxidative stress and down-regulate the levels of caspase-3 and bcl-2.