鸟嘌呤核苷酸抑制因子2基因表达与肝癌细胞迁移和侵袭能力的关系

Relationship between expression of RhoGDI2 gene and migration and invasion ability of hepatoma cells

目的:探讨鸟嘌呤核苷酸抑制因子2(RhoGDI2)对肝癌细胞迁移和侵袭能力的影响。方法:选取肝癌细胞株HCCLM3细胞,随机分为对照组、空白转染组和shRNA转染组,其中空白转染组转染空白质粒,shRNA转染组转染RhoGDI2-shRNA,采用MTT法检测各组细胞增殖,Transwell试验检测各组细胞迁移及侵袭能力,qRT-PCR检测各组细胞RhoGDI2 mRNA表达,Western blot检测各组细胞MMP-9、pAKT和VEGF表达。结果:shRNA转染组细胞RhoGDI2 mRNA相对表达量为(0.201±0.082),明显低于对照组和空白转染组(P<0.05);shRNA转染组细胞培养6 h、12 h和24 h时OD值分别为(0.312±0.110)、(0.562±0.107)和(0.700±0.110),明显低于对照组和空白转染组(P<0.05);shRNA转染组细胞迁移率和穿膜细胞数分别为(0.310±0.030)%和(9.28±1.11)个,明显低于对照组和空白转染组(P<0.05);shRNA转染组细胞MMP-9、pAKT和VEGF相对表达量分别为(0.762±0.100)、(0.201±0.092)和(0.501±0.101),明显低于对照组和空白转染组(P<0.05)。结论:下调RhoGDI2表达,可抑制肝癌细胞增殖、迁移及侵袭能力,值得进一步研究。

Objective:To investigate the effect of guanine nucleotide inhibitor 2(RhoGDI2) on the migration and invasion of hepatocellular carcinoma. Methods: HCCLM3 cells were randomly divided into control group,blank transfection group and shRNA transfection group,the blank plasmid was transfected in blank transfection group and RhoGDI2-shRNA was transfected in shRNA transfection group,MTT assay was used to detect cell proliferation,Transwell test was used to detect cell migration and invasion ability,qRT-PCR was used to detect RhoGDI2 gene expression,Western blot was used to detect the expression of MMP-9,pAKT and VEGF in each group. Results: The relative expression of RhoGDI2 in shRNA transfection group was(0.201±0.082),which were significantly lower than that in control group and blank transfection group(P<0.05);The OD values of shRNA transfection group at 6 h,12 h and 24 h were(0.312±0.110),(0.562±0.107) and(0.700±0.110),which were significantly lower than those of control group and blank transfection group(P<0.05);The cell migration rate and the number of transmembrane cells in shRNA transfection group were(0.310±0.030)% and(9.28±1.11),which were significantly lower than those in control group and blank transfection group(P<0.05)Conclusion:Downregulation of RhoGDI2 expression can inhibit the proliferation,migration and invasion of hepatocellular carcinoma cells,which deserves further study.