摘要
目的制定青黛的质量标准。方法对六种青黛样品进行性状鉴别和粉末显微鉴别;按药典方法分别测定其灰分、水分、水溶性浸出物和醇溶性浸出物含量;采用高效液相色谱法测定靛蓝、靛玉红含量。结果5号青黛样品为伪品,其余五种样品为真品。五种青黛水分含量平均值为5.9%,灰分含量平均值50.19%,酸不溶性灰分含量均值8.28%,水溶性浸出物含量平均值4.34%,醇溶性浸出物含量平均值1.97%,靛蓝平均含量2.16%,靛玉红平均含量0.17%。结论现行青黛质量标准的水分、靛蓝、靛玉红含量测定方法稳定可行;建议新增青黛总灰分含量、水溶性浸出物含量和醇溶性浸出物含量测定,以完善青黛的质量标准。
Objective To establish the quality standard of Indigo Naturalis.Methods Six samples of Indigo Naturalis were identified by morphology and powder microscopy.The contents of ash,water,water-soluble extract and alcohol-soluble extract were determined by pharmacopoeia method,and the contents of indigo and indirubin were determined by high performance liquid chromatography.Results The sample of Indigo Naturalis No.5 was fake and the other five samples were genuine.The average water content,ash content,acid-insoluble ash content,water-soluble extract,alcohol-soluble extract,indigo and indirubin contents were 5.90%,50.19%,8.28%,4.34%,1.97%,2.16%and 0.17%respectively.Conclusion The method for determination of water,indigo and indirubin in the current quality standard of Indigo Naturalis is stable and feasible.It is suggested that the examination of total ash content,water soluble extract content and alcohol soluble extract content should be added to further improve the quality standards of Indigo Naturalis.
作者
高思雨
白彩虹
王晓波
张旭
GAO Si-yu;BAI Cai-hong;WANG Xiao-bo;ZHANG Xu(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;The 967 Hospital of the Chinese People's Liberation Army,Dalian 116600,China)
出处
《中国医药指南》
2020年第12期35-36,共2页
Guide of China Medicine
基金
中国博士后科学基金资助项目(2016M603019)
辽宁中医药大学大学生创新项目(201610162101)。
关键词
青黛
质量标准
灰分
靛蓝
靛玉红
Indigo Naturalis
Quality standard
Ash
Indigo
Indirubin