miR-223-3p和LIF在子宫内膜异位症不孕患者子宫内膜组织中的表达意义

Expressions and significances of MiR-223-3p and leukemia inhibitory factor in endometrial tissue of infertile women with endometriosis

目的:探讨miR-223-3p和白血病抑制因子(LIF)在子宫内膜异位症(EMs)不孕患者内膜组织中的表达及意义。方法:选择2017年2月-2018年2月本院治疗的EMs并发不孕患者22例为异位症组,另选择育龄期非异位症月经正常妇女25例为对照组,采集两组黄体中期子宫内膜qRT-PCR法检测组织中miR-223-3p和白血病抑制因子(LIF)mRNA表达量,免疫组化法检测LIF蛋白表达,miRTarBase数据库预测miR-223-3p靶基因,Pearson法分析miR-223-3p与LIF相关性。结果:异位症组miR-223-3p mRNA表达水平高于对照组,LIF mRNA表达低于对照组(P<0.05);LIF的蛋白表达量异位症组(1.38±0.42)低于对照组(3.65±0.81)(P<0.05);miRTarBase数据库预测显示LIF基因上有miR-223-3p结合位点,Pearson相关性分析显示,异位症组miR-223-3p与LIF呈负相关(r=-0.625,P=0.002)。结论:EMs患者子宫内膜组织中miR-223-3p高表达,LIF低表达,二者负相关,miR-223-3p可能通过抑制LIF的表达,发挥对内膜组织调控作用。

Objective:To investigate the expressions and significances of miR-223-3p and leukemia inhibitory factor(LIF)in endometrial tissue of infertile women with endometriosis.Methods:22 infertile women with endometriosis were selected in study group from February 2017 to February 2018,and 25 married normal women with normal menstrual cycle were selected in control group.The endometrial tissue during the middle luteal phase of women in the two groups were collected.The expressions of miR-223-3p and LIF mRNA in endometrial tissue were detected by qRT-PCR,the expression of LIF protein in endometrial tissue was detected by immunohistochemistry,and the target gene of miR-223-3p was predicted by the microTarBase database,and Pearson correlation analysis was used to analyze the correlation between miR-223-3p expression and LIF level.Results:The expression of miR-223-3p of women in the study group was significant higher than that of women in the control group,and the expression of LIF of women in the study group was significant lower(P<0.05).The expression of LIF protein of women in the study group was 1.38±0.42,which was significant lower than that(3.65±0.81)of women in the control group(P<0.05).MicroTarBase database predicted that there were miR-223-3p binding sites on LIF gene.Pearson correlation analysis showed that miR-223-3p level of women in study group was negatively correlated with LIF level(r=-0.625,p=0.002).Conclusion:mir-223-3p expression in endometrial tissue of infertile women with endometriosis is high expression,but LIF expression is low,there is negatively correlated between the mir-223-3p expression and LIF expression.Mir-223-3p level may play a regulatory role in endometrial tissues by inhibiting the expression of LIF.