HIF-1α、VEGF、sFlt-1在子痫前期患者胎盘组织中的表达及临床意义

Expression of HIF-1α,VEGF and sFlt-1 mRNA in placenta tissue of patients with preeclampsia and their clinical significance

目的:探讨缺氧诱导因子-1α(HIF-1α)、靶基因血管内皮细胞生长因子(VEGF)、可溶性血管内皮生长因子受体1(sFlt-1)在子痫前期(PC)患者胎盘组织中的表达及临床意义。方法:将2016年6月-2018年6月本院收治的子痫前期患者120例作为观察组,选取同期正常妊娠妇女100例作为对照组。采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接法(SP)检测HIF-1α、VEGF、sFlt-1等水平,使用RT-PCR技术检测HIF-1α、VEGF、sFlt-1 mRNA水平并分析相关性。结果:观察组HIF-1α蛋白(+++)表达率(45.0%)高于对照组(12.0%),VEGF蛋白(+++)表达率(14.2%)低于对照组(47.0%),sFlt-1蛋白(+++)表达率(52.5%)高于对照组(20.0%)(均P<0.05);两组VEGF mRNA表达无差异(P>0.05);观察组HIF-1αmRNA、sFlt-1 mRNA高于对照组,VEGFm RNA/sFlt-1 mRNA比值低于对照组(均P<0.05);观察组HIF-1αm RNA与sFlt-1 mRNA呈正相关(r=0.578,P<0.05),与VEGF mRNA/sFlt-1 mRNA比值呈负相关(r=-0.382,P<0.05)。结论:HIF-1α、VEGF、sFlt-1与PC病理生理改变有关,可能参与了PC的发生发展。

Objective:To investigate the expression of hypoxia inducible factor-1 alpha(HIF-1 alpha),target gene vascular endothelial growth factor(VEGF),soluble vascular endothelial growth factor receptor-1(sFlt-1)in placenta tissue of patients with preeclampsia,and to study their clinical significance.Methods:120 pregnant women with preeclampsia were selected in observation group and 100 normal pregnant women were selected in control group from June 2016 to June 2018.Immunohistochemical streptomyces antibiotic protein-peroxidase ligation(SP)was used to detect the expression levels of HIF-1a,VEGF and sFlt-1.RT-PCR was used to detect the changes of HIF-1a,VEGF and sFlt-1 in placenta tissues of pregnant women in both groups,and the correlation among them was analyzed.Results:The expression rate of HIF-1 alpha protein(+++)and sflt-1 protein(+++)of women in the observation group were 45.0%and 52.5%,which were significant higher than those(12.0%and 20.0%)of women in the control group,but the expression rate of VEGF protein(+++)was 14.2%,which was significant lower than that(47.0%)of women in the control group(P<0.05).There was no significant different in VEGF mRNA expression level of women between the two groups(P>0.05).The levels of Hif-1 mRNA and sflt-1 mRNA of women in the observation group were significant higher than those of women in the control group,while the value of VEGFm RNA/sflt-1 mRNA of women in the observation group was significant lower(P<0.05).In the observation group,the hif-1 mRNA level of women was positively correlated with the sflt-1 mRNA level(r=0.578,P<0.05),but was negatively correlated with the value of VEGF mRNA/sflt-1 mRNA(r=-0.382,P<0.05).Conclusion:The changes of HIF-1α,VEGF and sFlt-1 levels maybe correlated with the pathophysiological of women with preeclampsia,which may play a role in the occurrence and development of preeclampsia.