摘要
将松针使用95%的乙醇提取,通过硅胶、RP-18、Sephadex LH-20色谱方法分离化合物,根据理化性质及波谱数据进行化合物的结构鉴定。采用MTT法,对化合物进行体外抑制癌细胞增殖的活性筛选,从五针松松针中分离得到8种化合物,化合物染料木黄酮、木栓酮和刺五加酮对胃癌细胞增殖具有一定的抑制作用。
The 95%ethanol was used to extract pine needles.The compounds were separated by silica gel,RP-18 and Sephadex LH-20 chromatography.The structure of the compounds was identified according to the physicochemical properties and spectral data.MTT method was used to screen the compounds for inhibiting the proliferation of cancer cells in vitro,and 8 compounds were isolated from the five-needle pine needles.
作者
李启照
孙维浩
LI Qizhao;SUN Weihao(School of Pharmacology, Xinhua College, Hefei 230088, China)
出处
《河南教育学院学报（自然科学版）》
2020年第1期38-41,共4页
Journal of Henan Institute of Education(Natural Science Edition)
基金
安徽教育厅自然科学重点研究项目“松针黄酮-叶酸-氧化石墨烯组装、微波释药抗胃癌作用机理研究”(KJ2017A627)
省级质量工程项目“GSP药品营销实验实训中心”(2016SXZX021)
校级质量工程项目“新华-华康校企共建实习基地”(2015XQJDX04)。
关键词
松针
结构鉴定
抑制
胃癌
癌细胞增殖
pine needles
structure identification
inhibition
gastric cancer
proliferation of cancer cell
