HPLC法同时测定外感风寒颗粒中10种成分

Simultaneous determination of ten constituents in Waigan Fenghan Granules by HPLC

目的建立HPLC法同时测定外感风寒颗粒(桂枝、防风、葛根等)中苦杏仁苷、3’-羟基葛根素、葛根素、3’-甲氧基葛根素、升麻素苷、升麻素、5-O-甲基维斯阿米醇苷、亥茅酚苷、肉桂酸、桂皮醛的含有量。方法该药物甲醇提取液的分析采用Agilent ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 m L/min;柱温25℃;检测波长207、254、280 nm。结果 10种成分在各自范围内线性关系良好(r≥0.999 1),平均加样回收率为96.97%~100.08%,RSD为0.80%~1.45%。结论该方法简便、重复性好,可用于外感风寒颗粒的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of amygdalin,3’-hydroxy puerarin,puerarin,3’-methoxy puerarin,prim-O-glucosylcimifugin,cimifugin,5-O-methylvisammioside,sec-O-glucosylhamaudol,cinnamic acid and cinnamaldehyde in Waigan Fenghan Granules(Cinnamomi Ramulus,Saposhnikoviae Radix,Puerariae lobatae Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 25℃thermostatic Agilent ZORBAX Eclipse XDB-C18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 m L/min in a gradient elution manner,and the detection wavelengths were set at 207,254,280 nm.RESULTS Ten constituents showed good linear relationships within their own ranges(r≥0.999 1),whose average recoveries were 96.97%-100.08%with the RSDs of 0.80%-1.45%.CONCLUSION This simple and reproducible method can be used for the quality control of Waigan Fenghan Granules.