摘要
为防治烟草马铃薯Y病毒(Potato virus Y,PVY)和烟草花叶病毒(Tobacco mosaic virus,TMV),筛选PVY和TMV基因组中保守且高效表达siRNA的片段,连接到烟草脉带花叶病毒(Tobacco vein banding mosaic virus,TVBMV)弱毒侵染性克隆,制备成单联弱毒疫苗,获得TVB-PVYF1、TVB-PVYF2、TVB-PVYF3和TVB-TMVF1、TVB-TMVF2、TVB-TMVF3共6个单联弱毒疫苗。室内筛选后选取保护效果最好的TVB-PVYF2和TVB-TMVF3两个单联弱毒疫苗在山东临沂、潍坊和黑龙江哈尔滨等地进行了田间防效测定,TVB-PVYF2对黑龙江哈尔滨、山东临沂和潍坊烟草PVY的相对防效分别为66.88%、76.46%和60.71%,TVB-TMVF3对黑龙江哈尔滨、山东临沂和潍坊烟草TMV的相对防效分别为80.74%、87.34%和74.40%。
To control Potato virus Y virus (PVY) and Tobacco mosaic virus (TMV),the conserved genomic sequences of PVY and TMV that have a great potential to produce siRNAs were screened and cloned to mild mutant vector of Tobacco vein banding mosaic virus (TVBMV),producing mild vaccines including TVB-PVYF1,TVB-PVYF2,TVB-PVYF3 and TVB-TMVF1,TVB-TMVF2,TVB-TMVF3.Primary tests showed that TVB-PVYF2 and TVB-TMVF3 had great potential on cross protection.Field tests were conducted in Harbin of Heilongjiang province,Linyi and Weifang of Shandong province.The results showed that relative control effects of TVB-PVYF2 against PVY in Harbin,Linyi and Weifang were 66.88%,76.46% and 60.71%,respectively,while those of TVB-TMVF3 against TMV in Harbin,Linyi and Weifang were 80.74%,87.34% and 74.40%,respectively.
作者
姜瀚林
田延平
郭兆奎
刘永中
万秀清
刘文涛
李现道
李向东
张永春
孟凡武
JIANG Hanlin;TIAN Yanping;GUO Zhaokui;LIU Yongzhou;WAN Xiuqing;LIU Wentao;LI Xiandao;LI Xiangdong;ZHANG Yongchun;MENG Fanwu(College of Plant Protection,Shandong Agricultural University,Tai’an 271018,Shandong,China;Heilongjiang Tobacco Research Institute,Harbin 1570076,China;Linyi Municipal Tobacco Company,Linyi 276003,Shandong,China;Shandong Provincial Tobacco Academy,Ji’nan 250098,China;Weifang Municipal Tobacco Company,Weifang 261061,Shandong,China)
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2020年第2期65-70,共6页
Acta Tabacaria Sinica
基金
黑龙江省烟草公司项目(HN201703),山东省烟草公司项目(KN238-201602),泰山学者建设工程(TS201712023)。
