应用CLSI EP7-A3评价肌酐测定的干扰因素

Evaluation of interference factors in creatinine measurement by using new guideline CLSI EP7-A3

目的:探讨美国临床实验室标准化协会(CLSI)EP7-A3在评价肌酐(Cr)测定干扰因素中的应用。方法:根据CLSI EP7-A3文件,采用实验当天新鲜血清(无溶血、脂血、黄疸),通过配对差异实验确认干扰物质,通过剂量效应实验中应用点对点分析方法明确干扰物质带来的结果差异。结果:甘油三酯16.94 mmol/L、盐酸多巴酚丁胺4.01μmol/L、抗坏血酸298μmol/L对Cr测定无干扰,以游离胆红素684μmol/L、结合胆红素684μmol/L、羟苯磺酸钙144μmol/L、血红蛋白10 g/L为最大干扰物浓度做剂量效应实验,结果显示以上干扰物对Cr测定有负干扰。结论:按照EP7-A3评价肌酐测定的干扰因素有一定的应用价值。

Objective To investigate the interfering factors in the determination of creatinine(Cr)using the American Clinical Laboratory Standards Association(CLSI)EP7-A3 document.Methods According to the CLSI EP7-A3 document,fresh serum(no hemolysis,lipemia,and jaundice)was used on the day of the experiment and confirmed the interfering substances through the pairing difference experiment and the point-to-point analysis method was used in the dose effect experiment to clarify the difference of interfering substances.Results Triglyceride(16.94 mmol/L),dobutamine hydrochloride(4.01μmol/L),ascorbic acid(298μmol/L)did not interfere with the determination of Cr.Free bilirubin(684μmol/L),conjugated bilirubin(684μmol/L),calcium hydroxybenzene sulfonate(144μmol/L)and hemoglobin(10 g/L)were used as the maximum concentrations of interferences for the dose effect test,the results showed that the above interferences had negative interference on the determination of Cr.Conclusion According to EP7-A3,it is valuable to evaluate the interference factors of creatinine determination.