受激拉曼散射显微技术用于快速无标记病理成像

Rapid Histological Imaging Using Stimulated Raman Scattering Microscopy

对病变组织进行快速成像以获取足够的诊断信息,可以帮助医生在术中做出重要决策。受激拉曼散射显微技术是一种新兴的无标记成像技术,利用生物分子自身的光散射性质,在不需要对组织进行任何处理的情况下即可获取类比于传统病理的成像结果。越来越多的研究表明受激拉曼散射显微技术作为一种"虚拟组织成像"工具,可以迅速区分病变与正常组织。这篇综述主要阐述了受激拉曼散射显微成像的基本原理、发展现状及其在组织成像上的应用。

Rapid histological imaging of pathological tissues with sufficient diagnostic information has great potential to aid doctors in intraoperative decision making. Stimulated Raman scattering(SRS) microscopy is an emerging label-free imaging modality capable of obtaining histological images of tissues without the need for time-consuming tissue processing, such as fixing, sectioning, and staining. An increasing number of studies have demonstrated SRS microscopy as a "virtual histology" tool for rapid diagnosis of various diseases. In this review, we focus on the basic principles and current developments of SRS microscopy as well as its applications for rapid tissue histology.