摘要
为了探明印度酸橘(Citrus reshni)脱落酸合成关键基因CrNCED1的功能,分析了CrNCED1的表达特性,并将其在柠檬中超表达获得转基因植株,对转基因植株进行脱水抗性分析。结果表明,CrNCED1在印度酸橘叶片中表达量最高;并且其表达受脱水、低温和ABA诱导,但不受盐诱导,其中ABA诱导上调作用强烈。超表达CrNCED1的转基因柠檬植株ABA含量显著高于野生型,脱水处理后叶片萎蔫程度比野生型轻,其相对失水率、MDA和活性氧含量均显著低于野生型,叶片气孔开度显著小于野生型,表明超表达CrNCED1增强了转基因柠檬的抗脱水能力。
To explore the function of Cr NCED1,a key enzyme gene during abscisic acid biosynthesis in Cleopatra Mandarin(Citrus reshni),the expression characteristics of CrNCED1 was analyzed.Transgenic plants were obtained by overexpression of CrNCED1 in lemon for assaying dehydration resistance.The results showed that Cr NCED1 showed the highest expression levels in leaves of Citrus reshni.Transcript levels of CrNCED1 were induced by dehydration,low temperature and ABA,but not by salt stress,among which ABA treatment strongly induced the expression of CrNCED1.The ABA content of transgenic lemon plants overexpressing CrNCED1 was remarkably higher than that of wild type.Upon exposure to dehydration treatment,the transgenic lines displayed less severe leaf wilting degree,concurrent with noticeably lower relative water loss,MDA and reactive oxygen species contents,and substantially smaller stomatal aperture in comparison with the wild type,indicating that overexpression of CrNCED1 conferred enhanced dehydration resistance in transgenic lemon plants.
作者
戴文珊
王敏
刘继红
DAI Wenshan;WANG Min;LIU Jihong(Key Laboratory of Horticultural Plant Biology,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430010,China;National Navel Orange Engineering Research Center,College of Navel Orange,Gannan Normal University,Ganzhou,Jiangxi 341000,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2020年第3期551-561,共11页
Acta Horticulturae Sinica
基金
湖北省自然科学基金创新群体项目(2017CFA018)
国家自然科学基金项目(3172273)。
