新型邻位诱导熵驱动策略用于幽门螺杆菌DNA的高灵敏检测

A novel strategy based on proximity binding-induced entropy-driven amplification circuits for high-sensitivity detection of Helicobacter pylori DNA

目的:构建新型邻位触及诱导熵驱动信号放大策略用于DNA的高灵敏检测。方法:靶DNA诱导邻位连接,与两亲和探针共同形成夹心复合物,从而形成邻位引物。所获得的邻位引物通过介导分支迁移点亮熵驱动放大环路以实现DNA的检测。结果:在最优实验条件下,本工作所制备的DNA传感策略显示出非常高的灵敏度和选择性,动态范围从0.01~100 nmol/L,检测限低至6.7 pmol/L。结论:这种快速、高性价比和高效率的信号放大策略为DNA检测提供了一个简单且灵敏的平台。

Objective:To establish a novel strategy based on proximity binding-induced entropy-driven amplification circuits for highsensitivity detection of DNA. Methods:Target DNA was used to trigger proximity binding to form a sandwich complex with two affinity probes and then a proximity trigger,which lit up the entropy-driven amplification circuits by mediating branch migration for DNA detection. Results:Under the optimal experimental conditions,the DNA sensing strategy established in this study showed high sensitivity and selectivity,with a wide dynamic range of 0.01-100 nmol/L and a limit of detection as low as 6.7 pmol/L. Conclusion:This rapid,cost-effective,and highly efficient signal amplification strategy provides a simple and sensitive platform for DNA detection.