RUNX3基因在急性髓系白血病中的表达及预后意义

Expression and Prognostic Significance of RUNX3 Gene in Acute Myeloid Leukemia

目的:探讨RUNX3(Runx transcription factors-3)在急性髓系白血病中的表达及临床预后意义。方法:利用GEPIA和UALCAN数据库分析RUNX3在急性髓系白血病以及各亚型中mRNA表达情况。对癌症基因组图谱(The cancer genomeatlas,TCGA)公共数据集中AML RNA-Seq数据采用Kaplan-Meier Plotter法对AML患者总生存期进行分析。利用UALCAN和String数据库对RUNX3相关基因及其蛋白互作网络和参与的生物学过程进行分析。结果:与正常外周血样本相比,RUNX3在急性髓系白血病患者外周血样本中高表达。在AML各亚型中,RUNX3在M0和M6中表达最高,在M4和M5中表达最低。RUNX3高表达的患者总生存期短(P=0.038)。此外,本研究发现,与RUNX3主要相互作用的蛋白是NOTCH1(P=0,R=0.62),PRF1(P=0,R=0.6),GZMB(P=1.7e-06,R=0.46)。主要参与的生物学过程有细胞黏附、小GTP酶介导的信号转导通路以及造血生成过程(P=0.0202)等。结论:RUNX3在AML中高表达,且在各亚型中表达水平存在差异。RUNX3高表达的AML患者预后较差。该研究为深入研究RUNX3在AML发生发展中的作用提供重要理论依据。

Objective:To explore the RUNX3(Runx transcription factors-3)expression in acute myeloid leukemia and its clinical prognostic significance.Methods:The mRNA expression of RUNX3 was analyzed in acute myeloid leukemia(AML)and each subtype by using the GEPIA and UALCAN databases.The overall survival of AML patients was analyzed by kaplan-meier Plotter method using RNA-seq data from AML samples in The Cancer Genome Atlas(TCGA)database.UALCAN and String databases were used to analyze RUNX3 related genes and their protein interaction networks,biological function enrichment and biological processes involved.Results:RUNX3 was highly expressed in the blood samples of patients with acute myeloid leukemia compared with normal peripheral blood samples.Analysis of RUNX3 expression in AML subtypes showed that RUNX3 expression was highest in M0 and M6,and lowest in M4 and M5.Patients with high expression of RUNX3 had a short overall survival(P=0.038).In addition,this study found that RUNX3 mainly interacts with NOTCH1(P=0,R=0.62),PRF1(P=0,R=0.6),and GZMB(P=1.7e-06,R=0.46).The major biological processes involved include cell adhesion,small GTp-mediated signal transduction pathway and hematopoiesis process(P=0.0202).Conclusions:RUNX3 is highly expressed in AML,and the expression level is different in each subtype.Patients with AML with high expression of RUNX3 have poor prognosis.This study provides an important theoretical basis for the in-depth study of the role of RUNX3 in the occurrence and development of AML.