正常新西兰白兔阴茎组织超声成像特征的实验研究

Experimental Study on Ultrasound Imaging of Normal New Zealand White Rabbit Penile Tissue

目的:明确正常新西兰白兔阴茎组织超声成像特征。方法:性成熟健康新西兰白兔3只(月龄4-5月),猝死后将阴茎切除放入4%中性福尔马林中固定24小时。将阴茎标本置入纯净水中,进行超声成像,扫查切面选择阴茎横截面。将阴茎标本横断面制成病理切片,进行HE染色观察。结果:超声成像清晰显示:阴茎包皮及皮下软组织、阴茎海绵体白膜、阴茎海绵体、尿道海绵体,这些结构的空间位置关系与阴茎标本和组织病理切片完全一致;同时,利用二维超声图像显示的白膜边界进行阴茎海绵体内径的测量,测值与组织病理切片基本一致。结论:利用二维超声可以观察和测量新西兰白兔阴茎组织结构,超声成像可以作为新西兰白兔阴茎疾病模型研究的一项影像学检测方法。

Objective: To identify the ultrasound imaging features of normal New Zealand white rabbits’ penile tissues. Methods:Three healthy New Zealand male rabbits(4-5 months) were euthanized, then the penis was separated and enclosed in 4.0% neutral formalin for 24 hours. The sample penis was placed in purified water for ultrasound imaging, with scanning the cross section of the cut penis.The sample penis was made into pathological sections and observed by HE staining. Results: It’s clearly observed and indicated using ultrasound imaging that the spatial positional relationships among penile foreskin and subcutaneous soft tissue, the tunica albuginea, the corpus cavernosum, and the urethral cavernosum are identical to the sample penis and histopathological sections. Consistently, the measured inner diameter of the corpus cavernosum using the two-dimensional ultrasound image with the tunica albuginea as the boundary,was almost equal to the value of the histopathological section. Conclusion: Two-dimensional ultrasound imaging can be used to observe and measure the penile tissue structure of New Zealand white rabbits. Method of ultrasound imaging can be used for the study of New Zealand white rabbits’ penile diseases model.