成年人鼻中隔软骨的成分分析

A compositional analysis of adult nasal septal cartilage

目的:定量定位研究成人鼻中隔软骨的主要生化成分,为临床进行鼻中隔软骨移植提供试验依据及理论指导。方法:取7具成人尸体鼻中隔软骨标本,每具均分为5个区域,标记为a、b、c、d、e区。采用生化分析测定鼻中隔软骨主要成分:软骨细胞、胶原蛋白和硫酸糖胺聚糖(s GAG)的相对湿重。结果:每mg湿软骨片段平均含有(22 900±2 900)个细胞(,60.3±6.4)μg胶原蛋白,sGAG的平均含量为每mg湿重(14.6±3.5)μg。软骨细胞数量和胶原蛋白在各区域差异无统计学意义(P>0.05)。与a区和c区比,b区和d区的s GAG含量显著增加(P<0.05)。与a区和c区比,b区、d区和e区胶原蛋白和sGAG的比值显著减小(P<0.05)。结论:sGAG的含量在鼻中隔软骨表现出区域特异性的变化。sGAG沉积的局部形态与鼻整形或鼻重建术中着重保留的L型支柱形状相一致。

Objective:To localize quantitatively the major biochemical constituents of human adult nasal septal cartilage,which may provide an experimental basis and theoretical guidance for clinical nasal septal cartilage transplantation.Methods:Seven adult corpse nasal septal cartilage specimens were obtained,each divided into 5 regions,labeled as A,B,C,D,and E respectively.Biochemical analysis was used to determine the relative wet weight of the main components of the septal cartilage:chondrocytes,collagen and sulfated glycosaminoglycans(sGAG).Results:Each milligram of wet cartilage fragment contains an average of(22900±2900)cells,(60.3±6.4)μg of collagen,and the average content of sulfated glycosaminoglycans(sGAG)was(14.6±3.5)μg per mg of wet weight.The number of cells and collagen did not change significantly in each region.Compared with region A and region C,the sGAG content in B and D regions was significantly increased(P<0.05).Compared with region A and region C,the ratio of collagen to sGAG in B,D and E regions was significantly decreased(P<0.05).Conclusion:The content of sGAG shows specific regional changes in the septal cartilage of the nasal septum.The local morphology of the sGAG deposition is consistent with the predominantly retained Lpillar shape in rhinoplasty or nasal reconstruction.