甘草酸对水飞蓟宾在大鼠体内药代动力学的影响

Effect of Glycyrrhizic Acid on Pharmacokinetics of Silybin in Rats

目的研究甘草酸对水飞蓟宾在大鼠体内药代动力学的影响。方法对大鼠分别灌胃水飞蓟宾(SLB)-甘草酸(GL)混悬液和水飞蓟宾(SLB)混悬液,采用高效液相色谱法测定大鼠血浆中水飞蓟宾浓度。用DAS 2.0软件处理数据,计算两组的药代动力学参数。结果SLB-GL混悬液组和SLB混悬液组的药代动力学参数Cmax分别为1.91μg·mL^-1和0.43μg·mL^-1,AUC(0→t)分别为5.45μg·mL^-1·h和1.91μg·mL^-1·h。与SLB混悬液组对比,SLB-GL混悬液组Cmax和AUC(0→t)分别增加344.19%和185.34%(P<0.05)。结论甘草酸可影响水飞蓟宾在大鼠体内的药代动力学过程,使得水飞蓟宾在大鼠体内的血药浓度和生物利用度升高。

OBJECTIVE To study the effect of glycyrrhizic acid on pharmacokinetics of silybin in rats.METHODS Rats were randomly divided into two groups,and given either silybin-glycyrrhizic acid suspension or silybin suspension via gastric gavage.HPLC method was used to determination the concentration of silybin in rat plasma.The pharmacokinetic profile of silybin was calculated by DAS 2.0 software.RESULTS The pharmacokinetic parameters of silybin in SLB-GL suspension group and SLB suspension group were as follows:Cmax-1.91 and 0.43μg·mL^-1,AUC(0→t)-5.45 and 1.91μg·mL^-1·h,respectively.Compared with SLB suspension group,Cmax and AUC(0→t)in SLB-GL suspension group increased by 344.19%and 185.34%,respectively.There was significantly different in Cmax and AUC(0→t)between the two groups(P<0.05).CONCLUSION The concentration and the bioavailability of silybin in rats can be increased by glycyrrhizic acid.