北京泛菌的生长特性及快速鉴定方法研究

Study on the growth characteristics and rapid identification methods of Pantoea beijingensis

[目的]研究分析了杏鲍菇软腐病病原菌北京泛菌的生长特性以及病原菌的快速鉴定方法,从而为杏鲍菇软腐病的防控打下基础。[方法]测定了北京泛菌在不同温度、不同种类抗生素、不同浓度的杀菌剂二氧化氯的生长情况,以及采用特异性引物进行PCR鉴定病原菌。[结果]北京泛菌生长的最适温度28℃,对硫酸链霉素、羧苄青霉素等7类抗生素敏感,在含有50 mg/L的杀菌剂二氧化氯的培养基中不能生长,引物对hrp LF/R、wzx CF/R、tua GF/R和lps DF/R在北京泛菌能够扩增出目的条带。[结论]明确了北京泛菌的生长特性,50 mg/L的杀菌剂能够有效杀灭病原菌,并开发了4对特异性引物用于病原菌的快速、准确鉴定,为杏鲍菇软腐病的防控打下基础。

[Objective]To lay the foundation for the prevention and control of soft rot disease of Pleurotus eryngii,the growth characteristics of pathogen Pantoea beijingensis and its rapid identification method was analyzed in this study.[Method]The growth curve of P.beijingensis at different temperatures,different kinds of antibiotics,and different concentrations of chlorine dioxide were measured.Specific primers were used to identify pathogenic bacteria by PCR.[Result]The optimal temperature of P.beijingensis was 28℃,and it was sensitive to 7 antibiotics.P.beijingensis cannot grow in the medium containing 50 mg/L chlorine dioxide.Primer pairs hrp LF/R,wzx CF/R,tua GF/R and lps DF/R can amplify target bands in P.beijingensis.[Conclusion]The growth characteristics of P.beijingensis were identified,50 mg/L chlorine dioxide can effectively kill pathogenic bacteria,and four specific primers were developed for rapid and accurate identification of pathogenic bacteria.The study laid the foundation for the prevention and control of soft rot disease to Pleurotus eryngii.