液相色谱-串联质谱法测定豆类中左旋多巴

Determination of L-DOPA in Beans by LC-MS/MS

文章建立一种豆类中左旋多巴的液相色谱-串联质谱测定方法。样品经0.3 mol/L乙酸水溶液超声提取,Agilent Proshell 120 SB-C18(2.1 mm×150 mm,2.7μm)色谱柱分离,以0.1%甲酸水-甲醇为流动相进行梯度洗脱,多反应监测(MRM),内标法定量。结果表明,左旋多巴在0.1~5.0 mg/L范围内线性关系良好,相关系数为0.9994,检出限为1.5 mg/kg,定量限为5.2 mg/kg,方法的平均加标回收率为96.9%~105.5%,日内精密度和日间精密度均小于4%,具有操作简单、灵敏度高、准确度高、重现性好等特点,能够很好满足豆类中左旋多巴含量的检测需要。

A liquid chromatography-tandem mass spectrometry method was developed for the determination of L-DOPA in Beans.The samples were extracted by 0.3 mol/L acetic acid solution with ultrasonic method and separated on an Agilent Proshell 120 SB-C18(2.1 mm×150 mm,2.7 μm) column by gradient elution using 0.1% formic acid solution-methanol as mobile phase.The L-DOPA was detected by multiple reaction monitoring(MRM) mode and quantified by internal standard method.The results showed that the correlation coefficient of the calibration curve was 0.9994 in the range of 0.1~5.0 mg/L,the limit of detection(LOD) was 1.5 mg/kg and the limit of quantitation(LOQ) was 5.2 mg/kg,the average recoveries ranged from 96.9% to 105.5%,the RSD values forthe intra-day and inter-day precisions were less than 4%The present method has advantages such as simplicity,sensitivity,accurately and could be applied to the determination of L-DOPA in beans.