基于焦平面估计的快速扫片方法

Fast Scanning Method Based on Multi-point Focusing

随着仪器自动化的发展,基于显微镜的自动阅片系统被广泛应用于医学领域。该系统需要在显微镜视野下移动多个位置并拍摄标本图像,以完成整个扫描任务。但是由于载物台平面与物镜中轴线不能保证绝对的垂直,或者观测样本表面的起伏,使得各个视野的焦点不在一个平面内,有必要通过聚焦移动以获得清晰图像。传统方法在每个位置都进行聚焦,导致聚焦移动次数过多,扫片的效率较低。为了减少扫片的时间,提出了一种基于焦平面估计的快速扫片方法。通过采集有代表性位置的焦点,估计其附近位置的焦点,在扫描载玻片时可以直接移动到估计的焦点位置完成聚焦。实验表明此方法能有效减少焦点搜索的次数,提高扫片效率。

With the development of instrument automation,the microscope-based automatic reading system is used widely in the medical field.This system needs moving many positions under the microscope and capturing the images to complete the scanning task.However,because it is difficult to ensure the plane of the platform and the axis of the object lens are vertical,and the surface of the observed samples may be not even,the focuses in different locations are not in a plane.Therefore,it is necessary to run auto focusing to obtain clear images.Conventional methods perform focusing at each location,causing excessive movements and inefficient scanning.In order to reduce the scanning time,this paper presents a fast scanning method based on focal plane estimation.By acquiring the focuses at several representative positions,the focuses of the position near them are estimated,and the platform(or the lens)can be moved directly to the estimated position.Experiments show that this method can reduce the number of focus searching effectively and improve the scanning efficiency.