矩阵式PCR扩增仪的设计

Design of matrix PCR instrument

针对PCR扩增仪的使用需求,设计了矩阵式PCR扩增仪。该扩增仪采用四个独立的孔反应槽,使用半导体制热制冷片对其升温和降温,每个反应槽均可单独设定工作温度。采用ARM微处理器作为系统的主控制器;围绕DS18B20数字温度传感器设计反应槽温度采集电路;以PID控制理论为基础,结合温度控制电路,对温度进行控制。反应槽温度控制范围0~100℃,精度为±0.5℃,升温速率最大可达5℃/s,每个反应槽均可单独设定12个温度点。经测试,该扩增仪达到了设计指标要求,可同时完成四批不同温度设定的基因扩增。

According to the need of PCR instrument,a matrix PCR instrument was designed. The instrument uses four independent pore reaction tanks,which are heated and cooled by a semiconductor heating and cooling sheet. Each reaction tank can be set with its own working temperature. ARM microprocessor is used as the main controller of the system;the temperature acquisition circuit of reaction tank is designed around DS18 B20 digital temperature sensor;and the temperature is controlled by PID control theory combined with the temperature control circuit. The temperature control range of the reaction tank is0~100℃,the accuracy is ±0.5℃,and the maximum heating rate can reach 5℃/s. each reaction tank can set 12 temperature points separately. After testing,the PCR instrument meets the design requirements,and can accomplish four different temperature-setting gene amplification at the same time.