广州市食品中蜡样芽胞杆菌生化分型和多位点测序分型分析

Biochemical typing and multilocus sequence type profiles of Bacillus cereus isolated from food in Guangzhou city

目的了解广州市食品中蜡样芽胞杆菌的生化分型、多位点测序分型(multilocus sequence type,MLST)情况和优势序列型。方法根据国标将79株食品来源蜡样芽孢杆菌进行生化分型,并根据PubMLST网站提供的蜡样芽胞杆菌MLST分型方案,扩增7个管家基因,经测序后,在PubMLST网站上比对获得菌株的序列型,利用eBURST V3和START2和MEGA6.0软件进行分析。结果广州市食品中的蜡样芽胞杆菌优势序列型为ST26,占16.46%。结论蜡样芽胞杆菌具有高度的遗传多样性。

Objective To study the biochemical typing,multilocus sequence type(MLST)profiles and predominant sequence type of Bacillus cereus(B.cereus)isolated from food in Guangzhou city.Methods The biochemical profiles of 79 strains of foofborne B.cereus were determined based on the national standard.Seven housekeeping genes were to be sequenced according to MLST scheme for B.cereus in PubMLST.The allele numbers and sequence types were provided after submission of housekeeping gene sequences to PubMLST.eBURST V3,START2 and MEGA 6.0 were employed for analysis of the MLST profiles.Results The predominant sequence type of B.cereus isolated from food in Guangzhou city was clone sequence type 26(ST26),accounting for 16.46%.Conclusions B.cereus isolated from food in Guangzhou shows a high genetic diversity.