微柱凝胶技术对于新生儿溶血病患儿输血前的检验效果

Effect of Microcolumn Gel Technique on the Test of Neonatal Hemolysis Before Transfusion

目的探讨微柱凝胶技术对于新生儿溶血病患儿输血前的检验效果。方法将南阳市骨科医院2017年3月-2018年9月收治的60例新生儿溶血病患儿(均进行输血治疗)作为本次研究,所有患儿的血标本均前后使用试管法、微柱凝胶技术检验血型,均前后使用聚凝胺法、微柱凝胶技术进行交叉配血,统计并分析60例患儿血标本的血型鉴定情况及交叉配血情况。结果微柱凝胶技术、试管法的正反定型不符率分别为1.67%、3.33%,差异无统计学意义(P>0.05);微柱凝胶技术、聚凝胺法交叉配血不合率分别为3.33%、13.33%,差异有统计学意义(P<0.05)。结论在新生儿溶血病患儿进行输血治疗前采用微柱凝胶技术检验,能准确检出患儿的血型,能有效检出交叉配血不合的情况,能提高临床输血的安全性,值得临床推广使用。

Objective To investigate the effect of microcolumn gel technique before blood transfusion in children with hemolytic disease of newborn. Methods 60 children with neonatal hemolytic disease in the hospital from March,2017 to September,2018,were treated with blood transfusion. All the blood samples were tested by test tube method,microcolumn gel technique,polycoagulant method and microcolumn gel technique before and after the whole treatment process. The blood group identification and cross-matching of blood samples from 60 children were statistically analyzed. Results The rate of positive and negative stereotyping of microcolumn gel technique and test tube method was 1.67% and 3.33% respectively,the difference was not statistically significant(P>0.05),and the cross matching rate of microcolumn gel technique and polycoagulant amine method was 3.33%and 13.33% respectively. The difference was statistically significant(P<0.05). Conclusion The microcolumn gel technique can accurately detect the blood group of the children with hemolytic disease of newborn before blood transfusion treatment,can effectively detect the cross matching blood incompatibility,can improve the safety of clinical blood transfusion,and is worth popularizing and using in clinic.