PCR荧光和PCR膜杂交检测HPV-DNA的比较研究

Comparative study of PCR-fluorescence and PCR-membrane hybridization in detection of HPV-DNA

目的:探索PCR-荧光法与PCR-膜杂交法在检测HPV-DNA中的对比研究.方法:收集某中心的208例标本,按照细胞组织学诊断分为5组,分别采用PCR-荧光法、PCR-膜杂交法检测HPV-DNA,比较两种方法检测的HPV-DNA阳性率.结果:恶性病变组采用两种方法检测HPV-DNA的阳性率是分别14.29%、28.57%(P<0.05).细胞异常组采用两种方法检测HPV-DNA的阳性率是92.59%、98.15%(P<0.05).结论:PCR-荧光法在临床诊疗筛查中应用效果很好,PCR-莫杂交法在非高危性HPV的检测中应用效果较好,PCR检测HPV-DNA具有很高的特异性和敏感性.

Objective:To explore the comparative study of PCR-fluorescence and PCR-membrane hybridization in detecting HPV-DNA.Methods:208 specimens were collected and divided into 5 groups.The positive rates of HPV DNA detected by PCR fluorescence and PCR membrane hybridization were compared.Results:The positive rate of HPV-DNA by PCR-fluorescence and PCR-membrane hybridization was 14.29%and 28.57%in patients with malignant lesions respectively(P<0.05).The positive rates of HPV-DNA in patients with abnormal cells detected by PCR-fluorescence and PCR-membrane hybridization were 92.59%and 98.15%(P<0.05).Conclusion:The application of PCR-fluorescence method in clinical diagnosis and treatment screening is very good.The application of PCR-Mohr hybridization method in non-high risk HPV detection is better.The detection of HPV-DNA by PCR has high specificity and sensitivity.