致奶牛乳房炎肺炎克雷伯氏菌的分离鉴定及防治建议

Isolation, Identification and Prevention and Control of Klebsiella Pneumoniae in Dairy Cattle

肺炎克雷伯氏菌为革兰氏阴性菌,是重要的条件性致病菌和医源性感染菌之一,也是引起奶牛乳房炎的重要病原菌。本研究对30份奶牛乳房炎乳样进行了病原菌的分离鉴定,并对分离菌进行药物敏感试验;结果表明,本次共分离肺炎克雷伯氏菌3株,遗传进化树关系显示分离菌株KP1、KP3与CP031810株、CP031562株、CP032185株亲缘关系较近,分离株KP2则相对较远。药物敏感试验结果显示,3株分离株均对阿莫西林、头孢他啶、头孢曲松耐药,对链霉素、庆大霉素表现敏感,分离菌株KP1和KP3对青霉素表现中度敏感,菌株KP2则表现耐药。本研究为奶牛场针对该菌的防控提供了基础数据。

Klebsiella pneumoniae is a gram-negative bacterium, which is one of the important opportunistic pathogens and iatrogenic infectious bacteria, and also an important pathogen of cow mastitis. This study isolated and identified pathogenic bacteria from 30 milk samples of cow mastitis, and conducted drug sensitivity test on the target bacteria. The results showed that 3 strains of Klebsiella pneumoniae were isolated, and the evolutionary tree relationship showed that the isolated strains KP1 and KP3 were closely related to CP031810 strains, CP031562 strains and CP032185 strains, while the isolated strains KP2 were far from each other. The drug sensitivity test results showed that the 3 isolates were all resistant to amoxicillin, ceftazidime and ceftriaxone, and sensitive to streptomycin and gentamicin. The isolated strains KP1 and KP3 showed moderate sensitivity to penicillin, while the isolated strains KP2 showed drug resistance. This study will provide basis for dairy farms for the prevention and control of the bacteria.